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A normalization strategy for comparing tag count data
Kadotaetal.AlgorithmsforMolecularBiology2012,7:5
/content/7/1/5
RESEARCH
OpenAccess
Anormalizationstrategyforcomparingtagcount
data
KojiKadota1,2*,TomoakiNishiyama3andKentaroShimizu1
Abstract
Background:High-throughputsequencing,suchasribonucleicacidsequencing(RNA-seq)andchromatin
immunoprecipitationsequencing(ChIP-seq)analyses,enablesvariousfeaturesoforganismstobecompared
throughtagcounts.RecentstudieshavedemonstratedthatthenormalizationstepforRNA-seqdataiscriticalfora
moreaccuratesubsequentanalysisofdifferentialgeneexpression.Developmentofamorerobustnormalization
methodisdesirableforidenti
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