A novel and universal method for microRNA RT-qPCR data normalization.docVIP

Open Access M d 2009MestdaghetVoal.lumeetho10, Issue 6, Article R64 A novel and universal method for microRNA RT-qPCR data normalization Pieter Mestdagh*, Pieter Van Vlierberghe*, An De Weer*, Daniel Muth?, Frank Westermann?, Frank Speleman* and Jo Vandesompele* Addresses: *Center for Medical Genetics, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium. ?Department of Tumour Genetics, German Cancer Center, Im Neuenheimer Feld 280, Heidelberg, Germany. Correspondence: Jo Vandesompele. Email: Joke.Vandesompele@UGent.be Published: 16 June 2009 Received: 2 April 2009 Revised: 2 Ap