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BatMeth improved mapper for bisulfite sequencing reads on DNA methylation
Limetal.GenomeBiology2012,13:R82
/2012/13/10/R82
METHOD
OpenAccess
BatMeth:improvedmapperforbisulfite
sequencingreadsonDNAmethylation
Jing-QuanLim1,ChandanaTennakoon1,2,GuoliangLi3,EleanorWong3,4,YijunRuan3,Chia-LinWei4,5 and
Wing-KinSung1,3*
Abstract
DNAmethylationplaysacrucialroleinhigherorganisms.Couplingbisulfitetreatmentwithnextgeneration
sequencingenablestheinterrogationof5-methylcytosinesitesinthegenome.However,bisulfiteconversion
introducesmismatchesbetweenthereadsandthereferencegenome,whichmakesmappingofIlluminaand
SOLiDreadsslowandinaccurate.BatMethisanalgorithmthatintegrates
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