Breeding of a new wastewater treatment yeast by genetic engineering.docVIP

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Breeding of a new wastewater treatment yeast by genetic engineering.doc

Breeding of a new wastewater treatment yeast by genetic engineering

KatoandIefujiAMBExpress2011,1:7 /content/1/1/7 ORIGINAL OpenAccess Breedingofanewwastewatertreatmentyeast bygeneticengineering MiyoshiKato1andHaruyukiIefuji1,2* Abstract WepreviouslydevelopedahostvectorsystemforthewastewatertreatmentyeastHansenulafabianiiJ640.The promoterandterminatorregionsofthegeneencodingglucoamylasefromH.fabianiiJ640wereusedforanew expressionvector,pHFGE-1.TheperformanceofpHFGE-1wascomparedwiththatofthewidelyusedpG-1 transformantvector.H.fabianiiJ640(HF-TAMY)cellsweretransformedwithpHFGE-1,andSaccharomycescerevisiae YPH-499(SC-TAMY)cellsweretransformedwithpG-1,bothofwhic

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