Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells.docVIP

ComparisonofEnzymaticandNon-EnzymaticMeans ofDissociatingAdherentMonolayersofMesenchymal StemCells BoonC.Heng,CatherineM.Cowan,andShubhayuBasu Abstract Thedissociationofadherentmesenchymalstemcell(MSC)monolayerswithtrypsinandenzyme-freedis- sociation buffer wascompared. Asignificantly lower proportion ofviable cells were obtained with en- zyme-free dissociation buffers compared totrypsin. Subsequently, thedissociated cells were re-seeded onnewcellculturedishesandweresubjectedtotheMTTassay24hlater.Theproportionofviablecells thatreattached wassignificantly lower forcells obtained bydissociatio