滑子菇多糖的结构分析.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.7 滑子菇多糖的结构分析 陈健，向莹 （华南理工大学,轻工与食品学院，广东广州 510640） 摘要：本文以滑子菇中分离纯化得到一种多糖PNP 为研究对象，通过高效凝胶渗透色谱法测定分子量及其分布，红外光谱、气 1 相色谱法、部分酸水解、高碘酸氧化法、Smith 降解法、甲基化分析、HNMR、氨基酸和β-消除反应分析多糖的结构，刚果红实验、 碘-碘化钾实验对该多糖溶液行为进行研究。分析表明，PNP 的重均分子量为20199 Da，含有木糖、甘露糖、葡萄糖和半乳糖这四种 单糖，且摩尔比为 1.5:3.36:14.2:1；经高碘酸氧化和 Smith 降解后表明，PNP 中没有1→4 糖苷键，而且 1→2 键:1→3 键:1→6 键为 1.42:5.06:1；PNP 具有典型的多糖红外吸收，糖链以β 型吡喃糖苷为主并且是一种含有蛋白质的糖缀合物的粘多糖；PNP 具有三股螺 旋结构，含有较长的侧链和分枝。 关键词：滑子菇；多糖；单糖组成；结构 文章篇号：1673-9078(2013)7-1544-1550 Structural Analysis of Polysaccharides from Pholiota nameko CHEN Jian, XIANGYing (School of Light Industry and Food Scicences, South China University of Technology, Guangzhou 510640, China) Abstract: A new polysaccharide PNP was isolated and purified from Pholiota nameko and its components and structure were studied. The PNP relative molecular mass and distribution were analyzed by high-performance gel permeation chromatography, the PNP main chain structure were determined by infrared spectra (IR), gas chromatography (GC), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation 1 and GC-MS, HNMR, amino acids and β-elimination reaction, and polysaccharide solution action was studied by Congo red and iodine-potassium experiment. The result indicated that the weight-average relative molecular mass of PNP was 20199 Da; PNP was composed of Xyl, Man, Glu and Gal with a molar ration of 1.5: 3.36: 14.2:1; through periodate oxidation and Smith degradation, the branches of PNP were composed of (1→2)-linked, (1→3)-linked and (1→6)-linked with a molar ration of 1.42: 5.06:1, and had no (1→4)-linked; PNP possessed