Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans.docVIP

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Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans.doc

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Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans

Huangetal.BiotechnologyforBiofuels2012,5:4 /content/5/1/4 RESEARCH OpenAccess Effectoforganicacidsonthegrowthandlipid accumulationofoleaginousyeastTrichosporon fermentans ChaoHuang1,HongWu1*,Zong-junLiu2,JunCai3,Wen-yongLou1andMin-huaZong1* Abstract Background:Microbiallipidshavedrawnincreasingattentioninrecentyearsaspromisingrawmaterialsfor biodieselproduction,andtheuseoflignocellulosichydrolysatesascarbonsourcesseemstobeafeasiblestrategy forcost-effectivelipidfermentationwitholeaginousmicroorganismsonalargescale.Duringthehydrolysisof lignocellulosicmaterialswithdiluteacid,however,variouskindsofinhibitors,especiallylargeamountsoforganic acids,willbeproduced,whichsubstantiallydecreasethefermentabilityoflignocellulosichydrolysates.Toovercome theinhibitoryeffectsoforganicacids,itiscriticaltounderstandtheirimpactonthegrowthandlipidaccumulation ofoleaginousmicroorganisms. Results:Inourpresentwork,weinvestigatedforthefirsttimetheeffectoftenrepresentativeorganicacidsin lignocellulosichydrolysatesonthegrowthandlipidaccumulationofoleaginousyeastTrichosporonfermentans cells.Incontrasttopreviousreports,wefoundthatthetoxicityoftheorganicacidstothecellswasnotdirectly relatedtotheirhydrophobicity.Itisworthnotingthatmostorganicacidstestedwerelesstoxicthanaldehydesto thecells,andsomecouldevenstimulatethegrowthandlipidaccumulationatalowconcentration.Unlike aldehydes,mostbinarycombinationsoforganicacidsexertednosynergisticinhibitoryeffectsonlipidproduction. Thepresenceoforganicacidsdeceleratedtheconsumptionofglucose,whereasitinfluencedtheutilizationof xyloseinadifferentandcomplicatedway.Inaddition,alltheorganicacidstested,exceptfuroicacid,inhibitedthe malicactivityofT.fermentans.Furthermore,theinhibitionoforganicacidsoncellgrowthwasdependentmoreon inoculumsize,temperatureandinitialpHthanonlipidcontent. Conclusions:Thisworkprovidessomemeaningfulinformationabouttheeffectoforganicacidinlignocellulosic hydrolysatesonthelipidproductionofoleaginousyeast,whichishelpfulforoptimizationofbi