Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms.docVIP

Sensors 2009, 9, 5503-5520; doi:10.3390/OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms María Pedrero, Susana Campuzano and José M. Pingarrón* Departamento de Química Analítica. Facultad de Ciencias Químicas. Universidad Complutense de Madrid. E-28040 Madrid, Spain; E-Mails: mpedrero@quim.ucm.es (M.P.); susanacr@quim.ucm.es (S.C.) * Author to whom correspondence should be addressed; E-Mail: pingarro@quim.ucm.es; Tel.: 34913944315; Fax: 34913944329 Received: 27 May 2009; in revised form: 2 July 2009 / Accepted: 10 July 2009 / Published: 13 July 2009 Abstract: The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered. Keywords: pathogen microorganisms; multiple detection; electrochemical sensors 1. Introduction The rapid and reliable detection and determination of pathogen microorganisms is of great importance nowadays, due to health and safety reasons. The main areas of research implied in this subject are the