Electrochemical Sensors for Detection of Acetylsalicylic Acid.docVIP

Sensors 2006, 6, 1483-1497 sensors ISSN 1424-8220 ? 2006 by MDPI /sensors Full Paper Electrochemical Sensors for Detection of Acetylsalicylic Acid Veronika Supalkova 1,2, Jiri Petrek 1, Ladislav Havel 1, Sona Krizkova 2, Jitka Petrlova 2, Vojtech Adam 2,3, David Potesil 2,3, Petr Babula 4, Miroslava Beklova 5, Ales Horna 6 and Rene Kizek 2,* 1 Department of Plant Biology, and 2 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 3 Department of Analytical Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic 4 Department of Natural Drugs, and 5 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic 6 Department of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, CZ- 762 72 Zlin, Czech Republic * Author to whom correspondence should be addressed; E-mail: kizek@sci.muni.cz Received: 16 October 2006 / Accepted: 27 October 2006 / Published: 6 November 2006 Abstract: Acetylsalicylic acid (AcSA), or aspirin, was introduced in the late 1890s and has been used to treat a variety of inflammatory conditions. The aim of this work was to suggest electrochemical sensor for acetylsalicylic detection. Primarily, we utilized square wave voltammetry (SWV) using both carbon paste electrode (CPE) and of graphite pencil electrode (GPE) as working ones to indirect determination of AcSA. The principle of indirect determination of AcSA bases in its hydrolysis on salicylic acid (SA), which is consequently detected. Thus, we optimized both determination of SA and conditions for AcSA hydrolysis and found out that the most suitable frequency, amplitude, step potential and the composition and pH of the supporting electrolyte for