Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors.docVIP

Sensors 2005, 5, 448-464 sensors ISSN 1424-8220 ? 2005 by MDPI /sensors Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors Libuse Trnkova1*, Frantisek Jelen2, Jitka Petrlova3, Vojtech Adam3, David Potesil3, Rene Kizek3 Dedicated to Professor Emil Palecek, on the Occasion of His 75th Birthday 1 Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic 2 Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic 3 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic * Author to whom correspondence should be addressed e-mail: libuse@chemi.muni.cz Received: 23 February 2005 / Accepted: 4 April 2005 / Published: 14 November 2005 Abstract: The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C) and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric  Sensors 2005, 5 449 curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS