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Expressed sequence tags (ESTs) and simple sequence repeat (SSR) markers from octoploid strawberry (Fragaria × ananassa).doc

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Expressed sequence tags (ESTs) and simple sequence repeat (SSR) markers from octoploid strawberry (Fragaria × ananassa)

BMC Plant Biology BioMedCentral Research article Open Access Expressed sequence tags (ESTs) and simple sequence repeat (SSR) markers from octoploid strawberry (Fragaria × ananassa) Kevin M Folta*?1, Margaret Staton?2, Philip J Stewart1, Sook Jung2, Dawn H Bies1, Christopher Jesdurai2 and Dorrie Main2 Address: 1Plant Molecular and Cellular Biology Program and Horticultural Sciences Department, University of Florida, Gainesville, FL, USA and 2Genetics, Biochemistry Life Science Studies, Clemson University, Clemson, SC, USA Email: Kevin M Folta* - kfolta@; Margaret Staton - meg@; Philip J Stewart - stewart5@; Sook Jung - sjung@; Dawn H Bies - dbies@; Christopher Jesdurai - jcdr@; Dorrie Main - dmain@ * Corresponding author ?Equal contributors Published: 28 June 2005 Received: 06 April 2005 Accepted: 28 June 2005 BMC Plant Biology 2005, 5:12 doi:10.1186/1471-2229-5-12 This article is available from: /1471-2229/5/12 ? 2005 Folta et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Cultivated strawberry (Fragaria × ananassa) represents one of the most valued fruit crops in the United States. Despite its economic importance, the octoploid genome presents a formidable barrier to efficient study of genome structure and molecular mechanisms that underlie agriculturally-relevant traits. Many potentially fruitful research avenues, especially large-scale gene expression surveys and development of molecular genetic markers have been limited by a lack of sequence information in public databases. As a first step to remedy this discrepancy a cDNA library has been developed from salicylate-treated, whole-plant tissues and over 1800 expressed sequence tags (ESTs) have been sequenced and analyzed. Result