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Expression and subcellular localization of aquaporin water channels in the polarized hepatocyte cell line, WIF-B.doc

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Expression and subcellular localization of aquaporin water channels in the polarized hepatocyte cell line, WIF-B

BMC Physiology BioMedCentral Research article Open Access Expression and subcellular localization of aquaporin water channels in the polarized hepatocyte cell line, WIF-B Sergio A Gradilone1, Pamela S Tietz2, Patrick L Splinter2, Raúl A Marinelli1 and Nicholas F LaRusso*2 Address: 1Instituto de Fisiología Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000 Rosario, Santa Fe, Argentina and 2Center for Basic Research in Digestive Diseases. Mayo Medical School, Clinic and Foundation, Rochester, MN, USA Email: Sergio A Gradilone - sgradilo@.ar; Pamela S Tietz - tietz.pamela@; Patrick L Splinter - Splinter.Patrick@; Raúl A Marinelli - rmarinel@.ar; Nicholas F LaRusso* - larusso.nicholas@ * Corresponding author Published: 18 August 2005 Received: 13 June 2005 Accepted: 18 August 2005 BMC Physiology 2005, 5:13 doi:10.1186/1472-6793-5-13 This article is available from: /1472-6793/5/13 ? 2005 Gradilone et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Recent data suggest that canalicular bile secretion involves selective expression and coordinated regulation of aquaporins (AQPs), a family of water channels proteins. In order to further characterize the role of AQPs in this process, an in vitro cell system with retained polarity and expression of AQPs and relevant solute transporters involved in bile formation is highly desirable. The WIF-B cell line is a highly differentiated and polarized rat hepatoma/human fibroblast hybrid, which forms abundant bile canalicular structures. This cell line has been reported to be a good in vitro model for studying hepatocyte po