Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana.docVIP

Molecules 2011, 16, 466-476; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Feeding Deterrents against Two Grain Storage Insects from Euphorbia fischeriana Zhu Feng Geng 1, Zhi Long Liu 2,*, Cheng Fang Wang 3, Qi Zhi Liu 2, Sheng Min Shen 3, Zi Mu Liu 3, Shu Shan Du 3,* and Zhi Wei Deng 1 1 Analytical and Testing Center, Beijing Normal University, Beijing 100875, China 2 Department of Entomology, China Agricultural University, Haidian District, Beijing 100094, China 3 Protection and Utilization of Traditional Chinese Medicine of Beijing Area Major Laboratory, Beijing Normal University, Haidian District, Beijing 100875, China * Authors to whom correspondence should be addressed; E-Mails: zhilongliu@ (Z.-L.L.); dushushan@ (S.-S.D.); Tel.: +86-10 Fax: +86-10 Received: 17 November 2010; in revised form: 5 January 2011 / Accepted: 7 January 2011 / Published: 10 January 2011 Abstract: The screening of several Chinese medicinal herbs for insecticidal principles showed that Euphorbia fischeriana roots possessed significant feeding deterrent activity against two stored-product insects (Tribolium castaneum and Sitophilus zeamais). From ethanol extract, four feeding deterrents were isolated by bioassay-guided fractionation. The compounds were identified as jolkinolide B, 12-deoxyphorbol 13-(9Z)-octadecenoate 20- acetate, 17-hydroxyjolkinolide A and B on the basis of their phytochemical and spectral data. Jolkinolide B and 17-hydroxyjolkinolide B possessed strong feeding deterrent activities against S. zeamais (EC50 = 342.1 and 543.9 ppm, respectively) and T. castaneum adults (EC50 = 361.4 and 551.5 ppm, respectively). 17-Hydroxyjolkinolide A and 12- deoxyphorbol 13-(9Z)-octadecenoate 20-acetate A also exhibited feeding deterrent activity against the two grain storage insects with EC50 values of 631.9 and 884.3 ppm for S. zeamais and 6