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Ferric Reducing Antioxidant Power and Square Wave Voltammetry for Assay of Low Molecular Weight Antioxidants in Blood Plasma Performance and Comparison of Methods.doc

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Sensors 2009, 9, 9094-9103; doi:10.3390/OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Ferric Reducing Antioxidant Power and Square Wave Voltammetry for Assay of Low Molecular Weight Antioxidants in Blood Plasma: Performance and Comparison of Methods Miroslav Pohanka 1,*, Hana Bandouchova 2, Jakub Sobotka 3, Jana Sedlackova 2, Ivana Soukupova 2 and Jiri Pikula 2 1 Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defence / Trebesska 1575, 50001 Hradec Kralove, Czech Republic 2 Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech Republic; E-Mails: bandouchovah@vfu.cz (H.B.); sedlackovaj@vfu.cz (J.S.); soukupovai@vfu.cz (I.S.); pikulaj@vfu.cz (J.P.) 3 Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 95, 532 10 Pardubice, Czech Republic; E-Mail: sobotka.jakub@seznam.cz (J.S.) * Author to whom correspondence should be addressed; E-Mail: rau@atlas.cz; Tel.: +420-973-251-519; Fax: +420-495-518-094. Received: 2 September 2009; in revised form: 7 October 2009 / Accepted: 22 October 2009 / Published: 17 November 2009 Abstract: The purpose of the present study was to employ two methods—square wave voltammetry (SWV) performed on screen printed sensors and ferric reducing antioxidant power (FRAP)—as suitable tools for the assay of low-molecular-weight antioxidants (LMWAs). LMWAs were assayed by both methods and the resulting data were statistically compared. Plasma samples from five Cinereous vultures accidentally intoxicated with lead were used to represent real biological matrices with different levels of LMWAs. Blood was collected from the birds prior to and one month after treatment with Ca-EDTA. SWV resulted in two peaks. The first