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人参皂甙rb1对脑缺血再灌注损伤半影区葡萄糖转运体1表达的影响_李方成
# 196 # 2005 222 2 Chin J Exp Surg, February 2005, Vol 22, o. 2
# #
Rb1
1
李方成 陶宗玉 刘安民 李军亮 吴中华 林吉惠
= / 1
( GLUT 1) Rb1 SD 42
,3 :Rb1 ( MA-
CO) , 1h 5 h ,(TTC) ,
;-(RT-PCR) GLUT 1 mR A ;
GLUT 1 Rb1
0(0.04? 0.01) %(0.02? 0.01)%; 3 GLUT 1mR-
A ( 0. 23? 0. 14) ( 0.55? 0.04) ( 0.79? 0. 19) ; PU (5. 1?
1.2)( 13. 1? 1.7) ( 19.0? 1.9) Rb1
,Rb1 GLUT 1
= ; ; ;
Effect of ginsenoside Rb1on the expression of GLUT1 in penumbra area after cerebral ischemia/ reper-
fusion injury in rats L I Fang-cheng , TA O Zong-y u, L I U A n-min, et al. Dep art ment of Neuro-
surgery, The Secon Af f iliate Hosp ital, Zhongshan University, Guangz hou 510120, China
= Abstract Objecti e To investigate the change of infarct size and the expression of GLUT 1 in
penumbra area after cerebral ischemia/reperfusion injury in rats and the effect of ginsenoside Rb1 on
them.Methods Forty-two male adult SD rats were randomly divided into 3 groups: sham-operated
group,the control group and the ginsenoside Rb1group.The model of middle artery occlusion ( MCAO)
in rats was established by using an intraluminal thread technique.The influence of ginsenoside Rb1on in-
farct size, neurologic deficit,the expression level of GLUT 1mR A and protein in the penumbrawere ob-
served.The infarct size was analyzed quantitatively by Kontron IBAS 2. 5 image auto-analyses system.
T he change of GLUT 1mR A was assessed by RT-PCR.T he expressionof GLUT 1protein was detected
by immunohistochemistry.Results The volume percentage of infarct in sham-operated group,the control
group and the ginsenoside Rb1 group was 0, (0.04? 0.01) % and (0.02? 0.01) % respectively ( P
0.01) .The level of GLUT 1 mR A in sham-operated group, the c
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