正交试验设计优选心脉安的初步提取工艺.pdfVIP

WWW. zgys. org 研究抢文 正交试验设计优选心脉安的初步提取工艺 3 刘春平1 ， 2 全向阳1 ， 2 乐智勇1 ， 2 魏冈tl ( 1.广东康美药物研究院有限公司 广州 51删6;2. 康美药业股份有限公 司 ;3. 广州中医药大学) 摘 要 目的:优选心脉安的最佳初步提取工艺。方法:以丹参嗣H 含量为指标，考察丹参脂溶性成分的提取工艺;以丹盼 A 酸B 含量为指标，采用正交试验优选丹参醇提后药渣与黄武、赤~、麦冬合并提取水溶性成分的录佳工艺。结果:丹参醇提取 最佳工艺为 6 倍量959串乙醇渗漉36 h; 水提取最佳工艺为 8 倍量水提取两次，每次1. 5h。结论:优选的提取方法可行，可为 心脉安进一步研究及产业化提供依据。 关键词 心脉安;提取工艺;正交试验;丹参嗣 llA; 丹盼酸B 中固分类号: R284.2 文献标识码:A 文章编号:1∞8-049X( 2014 )07-1134-05 Optimization of Preliminary Extraction Process for Xinmaian by Orthogonal Experiment 1 1 1 3 Liu Chunping ,2 , Quan Xiangyang ,2 ,Le Zhiyong ,2 , Wei Gang ( 1. Guangdong Kangmei Phannaceutical Research Institute Co. Ltd. , Guangzhou 51仪沁6 ， China; 2. Kangmei Phannaceutical Co. Ltd. ; 3. Guangzhou University of Chinese Medicine) ABSTRACT Objective: To optimize the preliminary extraction pr∞ess for Xirunaian. Methods: Ethanol percolation technology was used for the extraction of liposoluble constituents in Salviae Miltiorrhizae with the ωntent of tanshinone II A as the index. 币le orthogo- nal test was adopted to optimize the optimal extraction process of water-soluble components in the mixture of Astragali Radix , Halloysi- tum Rubrum , Ophiopogonis Radix and the ethanol-extracted residue of Salviae Miltio础izae with the content of salvianolic acid B as the index. Results: 白le best ethanol-extraction process of Salviae Miltiorrhizae was percolating the materials by 6 times , amount of 95% ethanol for 36h. 咀le optimum water-extraction process was as fol1ows: adding 8 times 缸nount of water into the materìals and extracting 2 times with 1. 5h per time. Conclusion: 白le optimized extraction process is feasible , which provide唱 the basis