油菜菌核病菌抗腐霉利菌株的生理生化特性及抗药机制初步分析.pdfVIP

2010 年 12 月 中国油料作物字报 2010 ,32(4) ;534 - 539 Chinese journal of oil crop sciences 油莱茵核病菌抗腐霉利菌株的生理生化 特性及抗药机制初步分析 陈夕军，曹敏娟，~L维立，王艳，童蕴慧，徐教友‘ (扬州大学雷艺与植物保护学院，江苏扬州 225栅) 摘要:通过室内紫外线和药剂诱导，获得多株高筑腐霉利油莱茵核病菌( Sckrotinia sckrotiorum) 突变体。与野 生亲本菌株相比，抗性突变株(EC到〉缸沁怜喜ImL) 在离糖( 40glL 蕉糖)和高盐( 5喜IL NaCI) 培养基上生长缓 慢，表明对高渗透压比较敏感;茵丝相对电导率较高，显示细胞膜透性精大，抱内电解员渗出较多。试验还发现，在 含药培养液中，亲本菌株茵丝内甘油大量合成和积累，增加462.45% ，而抗药突变株甘泊含量仅稍有增加或有所下 降，最明抗腐霉剥突变株对外界渗透皇的谓节能力较链。根据己报道组氨酸激酶(HK) 基层序踌设计特异弓i 物，经 PCR 扩增和测序，获得油莱茵核病菌HK基因保守区域序列 1 605bpo 抗、感腐霉利商株 HK 基离保守在域在第45 位、第81 位和第625 位碱圣不同，但编码的氨基酸一致。医此，泊莱茵核病菌Ä1腐霉科的抗性及抗药茵株的高渗敏 感性与 HK 基因保守区域碱基突变无关。 关键词:菌核病菌:腐主革和~;就药性:渗透压敏感性;组氨酸激羁基因保守区域 中图分类号:S435.654 文献标识码:A 文章编号:1∞7 -9084(2010)04 -0534-06 Physiological and biochemical features of rocymidone-r臼is臼nt mutants to Sclerotinia sclerotiorum and prelimina叮 study of li创istance m配ha曲理 CHEN Xi - jun , CAO Min - ju邸， KONG Wei -wen , WANG Y矶， TONG Yun - hui , XU Jing - you. ( College 01 Horticulture and Plant Protection , Yangzhou Vniversity , Yang动ou 225∞9 ， China) Abstract: Several procymidone-resistant mutants of Sclerotinia sclerotiorum causing rape stem rot were ob- tained by UV or procymidone induction. 白ley grew slower (ECso 8∞μgImL) than their parent on media contai- ning sucro由( 4OglL) or NaCI ( 5 g1L) , indicating that the mutants we陀 more sensÌtive 10 high osmotic stress. 宫廷 relative conductivity of mutant mycelia was higher than that of the parent , which led to cell electrolyte leakage. 百le glycerol in parent mycelia increased quickly and accumulatεd by 462.45% in procymidone media , while that of the mutant was hardly changed , which implied