青钱柳种质资源多样性SRAP初步分析.pdfVIP

基因组学与应用生物学.2011 年，第 30 卷，第 1 期，第 40-46 页 Genomics and Applied Biology,2011 ,Vo 1.30,No.l ,40-46 研究报告 A Letter 青钱柳种质资源多样性 SRAP 初步分析 周一肠袱香香事尚旭岚杨万霞方升佐 南京林业大学森林资源与环境学院，南京，210037 *通讯作者， XX且I@叼 摘 要 为了利用分子标记方法评价青钱柳种质资源的遗传多样性，本文对青钱柳DNA 的提取、SRAP 扩 增体系重要参数进行了优化，运用优化体系筛选多态性引物，并用 1 对引物对青钱柳9 个种源进行了遗传多 样性初步分析。研究结果建立了适于青钱柳 SRAP 的扩增体系:从 110 对 SRAP 引物中筛选出了 13 对多态 性引物，运用 1 对引物组合Me7+Em2 扩增获得到个多态性位点，多态率达 100%。遗传多样性分析表明有 效等位基因数(Ne)为1.3429，平均 Shannons 信息指数(1)为 0.368 7 ,Neis 基因多样性指数(因为 0.2267 ，种 源的遗传分化指数Gst 为 0.1983; 聚类分析结果表明 9 个种源在遗传距离0.100 处聚为 3 类，聚类结果和地 理距离之间呈现较高的相关性。本文的研究结果表明所建立的青钱柳种质资源库具有广泛的遗传多样性，为 进一步的开发利用提供了条件。 关键词 青钱柳， SRAP，优化，遗传多样性，聚类 Preliminary Study on the Genetic Diversity of Germplasm for Cyclocαyα p a1iurus Revealed by SRAP Markers Zhou Yiyang Fu Xiangxiang * Shang Xulan Yang Wanxia Fang Shengzuo College ofForest Resources and Environrnent,N叫ing Fores町University， Nanjing , 210037 * Co盯巳sponding author,xxfu@ DOI: 10.3969/gab.030.000040 Abstract In order to assess the genetic diversity of germplasm for Cyclocαryα paliurus by molecular markers , DNA extraction method and key parameters of SRAP reaction system were optimized; pol严no叩hic primers were screened with the optimal system, and preliminary analysis of genetic diversity of 9 provenances by using a pair of primers wωoperated in this paper. The results showed that a SRAP reaction system 岛r Cyclocαrya paliurus was established through optimal design. 13 pairs of polymorphic primers were screened 仕om 110 pairs of SRAP mark- ers and 21 bands were amplified using primer pair Me7+Em2 with the pol严no甲hic rate of 100%. Moreover, ge- netic diversity analysis showed that the mean Ne value of 9 provenances was 1.342 9; the mean 1value , the mean H value and provenance Gst was 0.368 7 , 0.226 7 and 0.1983 , respectively. The UPGMA clustering analysis dis- played that 9 provenances could be divided into 3 groups at the genetic dista