加味桃核承气汤对肝纤维化大鼠TGFβ1蛋白表达的影响.docVIP

加味桃核承气汤对肝纤维化大鼠TGFβ1蛋白表达的影响 作者：赵治友 邬亚军 张俊杰 林庚庭 周庚生 汪国运 【摘要】 [目的]观察加味桃核承气汤对四氯化碳肝纤维化大鼠肝组织转化生长因子β1(TGFβ1)蛋白表达的影响。[方法]用四氯化碳诱导大鼠肝纤维化模型，研究分组为正常对照组、模型对照组、加味桃核承气汤高、中、低剂量 治疗 组和秋水仙碱对照组;灌胃给药，疗程8周;用免疫组化技术检测肝组织TGFβ1的蛋白表达;检测血清ALT、AST水平;用苦味酸天狼红胶原染色方法进行半定量肝纤维化计分(SSS)。[结果]高、中、低剂量组及秋水仙碱组的TGFβ1的表达水平均低于模型对照组;经加味桃核承气汤治疗后血清ALT、AST水平及肝纤维化SSS计分显著下降。[结论]加味桃核承气汤可抑制肝纤维化大鼠TGFβ1的表达，达到抗肝纤维化作用。 【关键词】 加味桃核承气汤;四氯化碳;大鼠;肝纤维化 　　Abstract：[Objective]To study the effects of TGFβ1 of JiawEitaohechengtang on hepatic fibrosis induced by CCl4 in rats.[Methods]Hepatic fibrosis models were induced by CCl4 in rats.All the experimental rats were divided into six groups： normal control group，model control group，colchicine positive treatment group，highdose，mediumdose，lowdose jiawEItaohechenqitang treatment groups.Drugs were fed on rats for 8 weeks by intragastric administration.Protein expression levels of transfer growth factorsβ1(TGFβ1)were determined with immunohistochemical staining method.Scores of hepatic fibrosis were assessed with semiquantitive scoring system(SSS)through tianlanghong staining.[Results]The protein expression levels of TGFβ1 in the liver tissues in model control group were markedly increased compared with those in normal control group.Scores of hepatic fibrosis and the levels of serum ALT and AST in model control group were higher than those in normal control group，but lower than those in three treatment groups with jiaweitaohechengqitang.There were significant differences between model control group and three jiaweitaohechengqitang treatment groups.[Conclusion]Jiaweitaohechengqitang has therapeutic effects on hepatic fibrosis induced by CCl4 in rats. 　　Key words：jiaweitaohechengqitang;CCl4;rat;hepatic fibrosis 　 肝纤维化是各种不同原因所致慢性肝病的共同病理特征，任其 发展 可致肝硬化，抗肝纤维化治疗可阻断其向肝硬化进展。加味桃核承气汤在临床用于肝纤维化治疗中取得了很好的疗效，现对该方作深入研究，为临床推广应用该方提供实验依据。 　 　1 材料与方法 　　1.1 材料 (1)实验药品：加味桃核承气汤主要由桃仁、桂枝、大黄、甘草、莪术、白术、香附、茯苓等组成，用传统方法浸泡、煎煮、过滤、浓缩，制成煎剂，每毫升含生药2.7g，由浙江中医学院门诊部煎制;秋水仙碱：昆明制药集团股份有限公司。(2)实验动物：SD大鼠，150只，体重160～200g，雌雄各