茶树组培快繁技术的优化研究.pdf

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茶树组培快繁技术的优化研究

2005 25 3172~176 Journal of Tea Science * 310008 MS+BA 2.0+NAA 0.1+GA3 3.0 mg/L 30 d2.75 5 cm 20% S571.1; Q813.1+2 A 1000—369X200503—172—05 The Optimization Research on Tissue Culture and Rapid Propagation of Camellia Sinensis * ZHOU Jian, CHENG Hao , WANG Li-yuan (Key Lab of Tea Chemical Engineering, Ministry of Agriculture; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China) Abstract: The effect of different hormone on proliferation and growth of tea tissue culture seedlings were investigated, and an optimal culture medium of MS + BA 2.0 + NAA 0.1 + GA3 3.0 mg/L was screened out. On this optimal medium, the proliferation rate of tea cultured buds was 2.75 times after 30’s incubation. And more than 20% of those buds could developed to seedlings higher than 5 cm. Key words: Tea, Tissue culture, proliferation 80 [1] 2~3 [2] [3] [4] 43 2~3 [5] 0.1% HgCl2 2~3 -20 10 min 1 cm 3~4 1.2 1 1.1 1~3 2005—02—23 2005—07—04 1979— * E-mail: chenghao@ 3 173

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