_测定茶叶中27种有机氯和拟除虫菊酯农药多组分残留气相色谱法.pdf

DOI :10.13590/j.cjfh.2005.05.001 论著 27 蒋定国　方从容　杨大进　王竹天 (中国疾控中心营养与食品安全所, 北京　100021) 　:为了更有效地对我国茶叶的质量和安全性进行监 , 建立了一种快速测定茶叶中20 种有 机氯和7 种拟除虫菊酯农药多残留的气相色谱法。试样中农药残留在超声波振荡条件下用乙腈提 取, 提取液经氮气流浓缩和Florisil 固相萃取小柱净化, 用气相色谱-电子捕获检测器测定, 色谱柱 为HP-5 小口径石英毛细 柱(30 m×0.32 mm×0.25 μm)。农药的添加水平在6.0 ～228 μg kg 范 围时, 在绿茶中的平均添加回收率为71.2%～ 115 %, 相对标准差为3.42%～18.9 %;在花茶中的平 均添加回收率为71.1%～ 121%, 相对标准差为3.55%～ 19.2%。方法的检测限范围为0.12 ～6.0 μg kg。该方法快速、灵敏、准确、成本低, 能满足农药多残留快速扫描的分析要求。 :茶叶;色谱法, 气相;农药残留量;杀虫剂, 有机氯;除虫菊酯类 Determination of 27 organochlorine and pyrethroid pesticide multi-residues in teas by gas chromatography JIANG Ding-guo, FANG Cong-rong, YANG D -jin, WANG Zhu-ti n (N tion l Institute for Nutrition nd Food S fety, Chinese CDC, Beijing 100021, Chin ) Abstract :To control the pesticide residues in te , r pid nd sensitive method h s been est blished for the determin tion of 27 org nochlorine nd pyrethroid pesticide multi-residues in te s.The pesticide residues in te s mples were extr cted with cetonitrile nd by ultr sonic, the extr cts were concentr ted with stre m of nitrogen, cle ned up by Florisil solid ph se extr ction column, nd then determined by g s chrom togr phy with electron c pture detector.The column usedw s HP-5 c pill ry column (30 m×0.32 mm×0.25 μm). Twenty org nochlorine nd 7 pyrethroid pesticide multi-residues in te s were determined by this method.When pesticide residues in te s were t the 6.0 ～228 μg kg levels, the me n recoveries in green te s r nged from 71.2%to 115%with the rel tive st nd rd devi tion of 3.42%to 18.9%, nd the me n recoveries in floscul r te r nged from 71.1%to 121%with the rel tive st nd rd devi tion of 3.55%to 19.2%.The limits of detection of this method, b sed on chrom togr ms of bl nk s mples nd pesticide st nd rds,