分子与基因工程实验七.pptVIP

Transformation frequency Independent transgenic plants/inoculated immature embryo or callus. Line The seedlings from one transgenic cell, namely, one independent successfully transformation event, belong to one line, also are called clones. Generation These transgenic seedlings are T0 generation seedlings, T0 generation mature seeds are T1 generation…Meiosis or pollination is the boundary of different generations. 实验七 农杆菌介导的叶盘法转化 目的及要求： 通过叶盘法将带有报告基因的农杆菌转入烟草中，诱导获得转基因植株。 了解农杆菌转化植物的原理，掌握农杆菌的培养和侵染植物的方法。 二、实验原理 农杆菌重组子中携带经改造的Ti 质粒（含有帮助T-DNA跳到植物染色体上的Vir 区）和包含T-DNA的双