反相高效液相色谱法测定壳聚糖纳米粒中丙酮酸乙酯的含量.docVIP

反相高效液相色谱法测定壳聚糖纳米粒中丙酮酸乙酯的含量 徐凤娥，赵兵新，孙树茂，孟永春，南开辉，俞阿勇，瞿? 佳 温州医科大学附属眼视光医院，浙江省温州市? 325000 Determination of ethyl pyruvate in chitosan nanoparticles by reversed-phase high-performance liquid chromatography Xu Feng-e, Zhao Bing-xin, Sun Shu-mao, Meng Yong-chun, Nan Kai-hui, Yu A-yong, Qu Jia The Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China 摘要? 背景：有关丙酮酸乙酯含量检测方法的研究较少，采用反相高效液相色谱法检测丙酮酸乙酯含量的文献更少。目的：建立测定壳聚糖纳米粒中丙酮酸乙酯含量的反相高效液相色谱法。方法：采用Agilent1200系列高效液相色谱仪检测丙酮酸乙酯-壳聚糖纳米粒中丙酮酸乙酯的含量。色谱柱：ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5 μm)，柱温25 ℃，流动相为乙腈-水(体积比为40∶60)，流速1 mL/min，检测波长210 nm，进样量20 μL。结果与结论：丙酮酸乙酯峰与辅料及溶剂峰分离良好，丙酮酸乙酯质量浓度在1-100 mg/L内于峰面积线性关系良好(r =0.999 6)，低、中、高浓度丙酮酸乙酯对照品溶液日内、日间精密度的相对标准偏差均小于3%，重复性实验的相对标准偏差为1.25%，稳定性实验的相对标准偏差为1.3%。3批样品的加样回收率分别为(91.5±1.0)%，(93.5±0.2)%，(94.4±0.4)%；包封率分别为(87.20±0.22)%，(90.50±0.15)%，(91.10±0.17)%。不同批次样品丙酮酸乙酯含量检测结果的相对标准偏差分别为0.9%，0.5%，0.3%。说明反相高效液相色谱法灵敏度高、线性范围宽、专属性强、精密度高、加样回收率好、结果精确可靠，可用于壳聚糖纳米粒中丙酮酸乙酯含量的测定。 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： 关键词?：?生物材料；?纳米材料；?药物控释材料；?壳聚糖纳米粒；?丙酮酸乙酯；?反相高效液相色谱；?包封率；?相对标准偏差； ?? Abstract： BACKGROUND: Research on ethyl pyruvate detection methods is reported rarely, and moreover, literature about reversed-phase high-performance liquid chromatography (RP-HPLC) for detection of ethyl pyruvate is less.OBJECTIVE: To establish an RP-HPLC method for determination of ethyl pyruvate in ethyl pyruvate-chitosan nanoparticles.METHODS:?The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm×150 mm, 5 μm) at 25 ℃, with the mixture of acetonitrile and water (40:60, V/V) as the mobile phase at the flow rate of 1 mL/min. The determination wavelength was set at 210 nm and the injection volume was 20 μL.RESULTS AND CONCLUSION:?The peak of ethyl pyruvate and the peaks of auxiliary materials and solvent were separated well. The linear rang of ethyl pyruvate was 1-100 mg/L (r=0.999 6). The relative standard deviation of both the i