斑马鱼母源凝集素基因zfol的克隆和表达分析.pdfVIP

第 3 5 卷第 3 期 中南民族大学学报（自然科学版） Vol.35 No. 3 2 0 1 6 年 9 月 Journal of South-Central University for Nationalities( Nat. Sci. Edition) Sep. 2016 斑马鱼母源凝集素基因的克隆和表达分析 王 红 莹 ，杜 熳 (中南民族大学生命科学学院，武陵山区特色资源植物种质保护与利用湖北省重点实验室，武汉430074) 摘 要 为验证凝集素Zfol是否为母源蛋白及其具体表达模式，揭示凝集素这类来源于母体的免疫物质在鱼类胚 胎发育早期的免疫保护作用并提供理论依据，克 隆 Zfol 的基因全长进行了生物信息学分析.利用R T -P C R 和 Western blot分析了 Z/〇Z m R N A 和 Zfol蛋白的时空表达.结果表明 :Z/〇Z m R N A 及 Zfol蛋白在斑马鱼胚胎发育过程中 的表达均呈动态变化. ？/〇Z m R N A 在成鱼的卵巢、心脏、肝脏、肾脏、脑中表达，在精巢中未检测到表达.Zfol蛋白在 精巢、心脏、脑、肝脏等组织中检测不到表达，而在肾脏中有低含量表达，在卵巢中有高含量表达. 关键词凝集素;斑马鱼；基因？ 克隆;表达分析 中图分类号S965.299 文献标识码 A 文章编号 2016)03~0026-04 Molecular Cloning and Characterization of Maternal Lectin Gene zfol in Zebrafish W ang H o n gyin g， Du M an ( Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China , College of Life Sciences, South-Central University for Nationalities of Life Science, Wuhan ,430074 ) Abstract To investigate the expression pattern of maternal lectin Zfol, and to provide the theoretical basis for immune protection effect of lectins from the mother^ immune material on embryogenesis, the full-length of zfol gene was cloned and the expression pattern was characterized. Bioinformatics analysis to zfol was also performed. The spatial-temporal expression pattern of zfol mRNA and protein levels was determined by RT-PCR and Western blot, respectively. The results indicated that both zfol mRNA and Zfol proteins showed dynamic expression pa