百里醌对兔骨关节炎模型关节软骨的保护作用及机制.PDFVIP

浙江医学2017 年第39 卷第6 期 荫论 著 百里醌对兔骨关节炎模型关节 软骨的保护作用及机制 钟甫华 江彬锋 曾宇晴 黄恺 陆建伟 【摘要 】 目的 探索关节腔内注射百里醌对兔骨关节炎模型关节软骨的保护作用及机制。方法 选用新西兰大白兔构建动 物模型，分为百里醌组、骨关节炎组和假手术组各20 只，观察并记录实验大白兔6min 内步行距离。术后9 周处死动物取左膝关节标 本进行大体组织学Gross 评分，切片行苏木精- 伊红（HE ）染色、番红染色后进行组织病理学Mankin 评分，采用RT-PCR 检测3 组 大白兔关节软骨中基底金属蛋白酶 -13（MMP-13 ）、基底金属蛋白酶抑制剂 -1（TIMP-1 ）、聚集蛋白聚糖（Aggrecan ）和2 型胶原 （Collagen Ⅱ）等靶基因表达水平。 结果 百里醌组大白兔的行走距离明显大于骨关节炎组（P＜0.05 ）；而Gross 评分、Mankin 评分 均低于骨关节炎组（均P＜0.05 ）。与骨关节炎组比较，百里醌组MMP-13mRNA 表达水平明显下降（P＜0.05 ），TIMP-1、Aggrecan 和 Collagen ⅡmRNA 表达水平明显上升（均P＜0.05 ）。 结论 百里醌对兔骨关节炎模型的关节软骨具有保护作用，可以减少疼痛，延缓 骨关节炎的进展。百里醌可能通过下调MMP-13mRNA 表达，上调TIMP-1、Aggrecan 和Collagen ⅡmRNA 表达的分子机制实现保护 作用。 【关键词】 百里醌 骨关节炎 动物模型 Protective effects of thymoquinone on cartilage in rabbit osteoarthritis model China 【Abstract 】 Objective To evaluate the effects of intra-articular injection of thymoquinone on cartilage in rabbit osteoarthritis model. Methods Sixty New Zealand rabbits were randomly divided into treatment group, model group and control group with 20 in each group. Knee osteoarthritis model was established in thymoquinone and model groups, and sham operation was performed in control group, intraarticular injection of thymoquinone was given to treatment group. The walking distance within 6min was observed and recorded. Rabbits were sacrificed and samples of left knees were collected 9 wks after operation. Gross scores, HE staining, safranin O staining and Mankin score were evaluated. Real-time polymerase chain reactive (PCR) was performed to analysis the mRNA level of MMP-13, TIMP-1, aggrecan, collagen Ⅱin cartilage tissue. Results Compared with model group, the thymoquinone group showed better pain rel