非模式生物转录组_刘红亮技术总结.pdfVIP

HEREDITAS (Beijing) 2013 8 , 35(8): 955―970 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2013.00955 非模式生物转录组研究 刘红亮, 郑丽明, 刘青青, 权富生, 张涌 , , 712100 转录组代表细胞或组织内全部的RNA 转录本(RNA transcripts), 反映不同生命阶段、不同组织类型、不 同生理状态以及不同环境条件下表达的基因。转录组研究可以从整体水平上反映细胞中基因表达情况及其调控 规律。非模式生物(Non-model organism)具有许多模式生物不具备的特征, 其转录组研究对解决基因进化、遗传 育种以及生态等诸多方面的问题具有重要意义。但由于非模式生物缺乏参考基因组信息, 传统的转录组研究方 法操作复杂, 实验周期长, 花费大, 使得其转录组研究进展缓慢。新一代高通量 RNA 测序技术(RNA-seq)完全 改变了转录组学的研究模式, 迅速成为研究非模式生物转录组的先进技术。文章详细论述了近年来利用 RNA-seq 技术进行非模式生物转录组研究的进展情况, 从样品准备、高通量 DNA 测序以及生物信息学分析等 方面介绍了利用 RNA-seq 技术研究非模式生物转录组的一般流程及方法, 并对其中有待进一步研究的问题进 行了展望。 转录组; 非模式生物; RNA-seq Studies on the transcriptomes of non-model organisms LIU Hong-Liang, ZHENG Li-Ming, LIU Qing-Qing, QUAN Fu-Sheng, ZHANG Yong Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest AF University, Yangling 712100, China Abstract: The transcriptome represents the whole complement of RNA transcripts in cells or tissues and reflects the expressed genes at various life stages, tissue types, physiological states, and environmental conditions. Transcriptome analysis provides a comprehensive understanding of gene expression and its regulation. Non-model organism has many interesting traits of which model organisms lack, and the study of its transcriptome has great significance in solving the questions of genetic evolution, genetic breeding, ecology and so on. Because of absence of reference genome information, and traditional transcriptome research methods which are complicated to operate, long experimenta