大剂量5-FU致肠黏膜严重损伤时肠上皮细胞PCNA的表达.pdfVIP

.1860. 南方医科大学学报σSouth Med Univ) 2007;27(12) 大部量5-FU 致肠黯膜严重损伤时肠上皮细胞PCNA 的表达 罗育其 l ，吴承堂 1 揭英]，廖康雄气张军在 2( 南方医科大学南方医院 l 普i是外科，2 手术室，广州1 510515) 搞要:吕的探讨大剂量 5-FU 致肠辈在膜严重损伤时肠上皮细胞增殖细胞核抗原(PCNA) 的表达及其意义。方法成年 C57BU6J 小鼠40 只，分为对照组(n=韵和实验组(n=32)o 对照组给予接腔注射 PBS ，实验结给予腹应注射大剂量 5-FU (1 50 mg!kg.d ，连续五出，分别于处理后第 1 ， 3 ， 5 天，剖杀小鼠取小肠，常规 HE 染色，免疫组化检测 PCNA 的表达。结 果与对照组比较，腹腔注射大剂量 5阳后，小鼠肠悉膜遭受严重破坏，翁膜萎缩，绒毛、隐窝结构消失;免疫组化结果 显示 PCNA 表达增加.PCNA 指数显著增高(PO.OI) 。结论腹腔注射大剂量 5-FU 后，肠军在膜细胞中表达 PCNA 的弱上 皮细胞比例增加，可能与肠黎膜损伤后修复有密切的关系。 关键词:肠慈膜;场上皮子细胞;PCNA;5-FU 中00 分类号:R969 文献标识码:A 文章编号: 1673-4254(2007)12-1860-03 Expression of proliferating cell nuclear antigen in severely damaged small intestinal mucosa due to high-dose 5-FU exposure 1 2 LUO Yu-qi ,WU Cheng-tang , WEN Ying , LIAO Kang-xiongl, ZHANG Jun-hua Dep缸位lent ofGeneral Surgery，变到peration Room , Nan也ng Hospital , Southem Medical University, Guangzhou 510515, China Abstract: Objective To detect the expression of proli也rating cell nuclear antigen (PCNA) in severely damaged intestinal mucosa due 10 high-dose 5-FU exposure. Methods Th泣ty-two adult C57BU6J mice were subjected to daily intraperitoneal high-dose 5-FU injection at 150 mg!kg for 5 consecutive days , and on days 1, 3, and 5，也e mice were sacrificed to obtain the small i豆testinal tissue for HE straining and in1munohistochemis可 for detecting PCNA expression. Another 8 mice wíth íntraperitoneal PBS injection served as 也c ∞ntrol group. Results High曲dose 5-FU exposure of thξmíce resulted in severe intestinal mucous damage，时也 complete destruction ofthe villi and c巧Pts and significant1y increased cells positive for PCNA expression (P0.01). Conclusions High-dose 5-FU trea加ent c皿 si伊ificantly increase the PCNA index, and the cells