牛血清在细胞造就基中的主要功效(国外英文资料).doc

牛血清在细胞培养基中的主要功能 Bovine serum is the largest natural medium in cell culture, and contains the nutrient content of rich cell growth, which is of great importance. The provision of hormones that maintain the growth of the cell index, which has no or little nutrients in the base medium, and the main low-molecular nutrients. Provide binding proteins that recognize vitamins, lipids, metals, and other hormones, and can combine or adjust the material energy they combine. In some cases, a combination of protein and toxic metal and hot raw material can be used to detoxify. It is the source of the factors needed for the cell wall and the spread of the plastic substrate. Start the acid alkalinity buffer. Provide protease inhibitor, which causes residual trypsin to be lost in cell transmission and protect cells from injury. The main components of cow serum Serum is a complex mixture that is largely unknown, but part of it is not yet clear, and the serum is made up and the content is constant The sex, age, physiological condition and nutritional condition of the donor were different. Protein is the main ingredient in cow serum. In addition to the fact that you can carry metal ions, fatty acids, and your own hormone protein, white protein, globulin. Fibrous adhesion cells promote cell adhesion; Alpha 2 macroglobulin inhibits the role of trypsin; The fetal bovine serum contains fetal globulins that promote cell adhesion; Transferrotein can combine iron ions to reduce toxicity And theyre used by cells. Peptides: platelet promoting growth factor can promote cell division and is one of the main members of the polypeptide family, which is the main pro-cell proliferation factor; fibroblasts Growth factor, epidermal cell growth factor, nerve cell growth factor and so on, the content in serum is very little, but it also has certain effect to cell growth. Hormones: the role of hormones in cells is manifold. Insulin: promoting cell uptake of glucose and amino acids is associated with cell