茚三酮比色法定量检测赖氨酸条件的研究_刘飞飞.pdfVIP

刘飞飞, 李群, 于岚 ( , 266071) : - , , pH, : pH 60, 10mL, 15m in, 10m in, 570nm : ; ; ; : T S2012 : A : 1006- 25 13( 20 10) 05- 0223- 03 Research of determ ination of lys ine by n inhydrin co lo rmi etry LIU Feife,i LI Qun, YU Lan ( Co llege of Chem ist y Chem ical and Env i onm enta l Eng inee , Q ingdao Un ive sity, Q ingdao 266071) Abstract: A cco d ing to the co lo mi et y p inc ip le of- am ino ac id and n inhyd in, the m ethod of quantitativ e dete m i nation of lysine by n inhyd in co lo mi e t y was esea ched, and the pH value of the so lu tion, dosage of the ninhyd in, heated tmi e and coo led tmi e w e e investigated in o de to find the optmi um conditions of dete m ination of lysine by n in hyd in colo mi et y. The optmi un cond itions w e e as fo llow s: the pH value w as 60, and the ninhyd in was added 10mL, heated 15m in and coo led 10m in, fina lly dete m ined a t 570nm. K ey words: lysine; ninhyd in; colo mi et y; content dete m ination [ 8] , pH , , [ 1~ 3] , , , , [ 4, 5] , , 1 [ 6, 7] , 11 , AR, ;