低剂量环磷酰胺联合人参皂甙Rg3_省略_制小鼠肺癌肿瘤血管生成的实验研究.pdfVIP

·730 · 2005 8 25 8 CJITWM, August 2005, Vol.25, No.8 ·博士之窗· Rg3 1 1 2 1 康欣梅 张清媛 佟丹丹 赵文辉 研究持续低剂量环磷酰胺(cyclophosphamide, CT )联合人参皂 Rg3 对肺癌肿瘤血管 生成的影响, 观察其抑瘤效果、毒副反应及生存期。 以荷Lewis 肺癌的C57/BL6 小鼠为模型, 随机分 为5 组:即低剂量CT 组(简称低CT 组)、最大耐受剂量CT 组(简称高CT 组)、人参皂 Rg3组、低剂 量CT 联合人参皂 Rg3组(简称联合组)及模型组, 治疗中观察肿瘤体积、小鼠体重和外周血白细胞计数 及小鼠生存期, 测定肿瘤微血管密度(microvascular density, MVD)和血管内皮生长因子(vascular endothelial growth factor, VEGF)表达情况。 低CT 组肿瘤生长比较缓慢,小鼠体重及外周血白细胞数均无明 显下降,生存期延长;联合组抑瘤效果更持久而稳定,毒副反应未增加,小鼠生存期更长(P0.01)。低CT 组较高CT 组MVD 下降(P0.05);与模型组比较,低CT 组和人参皂 Rg3 组MVD 及VEGF 表达均 下降,两者合用时下降更明显(P0.05)。 低剂量CT 与人参皂 Rg3 联合应用显示出明显的抗血 管生成协同作用, 抑瘤效果显著且持久,毒副反应小,生存期较单药治疗延长。 环磷酰胺;人参皂 Rg3;血管生成;肺肿瘤 Experimental Study on Anti-angiogenesis in Mice with Lewis Lung Carcinoma by Low-dose of Cyclophos- phamide Combined with Ginsenoside Rg3 KANG in-mei,ZHANG Qing-yuan, TONG Dan-dan,et al.Dep ar t - men t of I nter na l M ed icine, Af f ilia ted Hosp i ta l of Oncology , Harbin Medica l Un iversity ,H ar bin (15 0040 ) Abstract　Objecti e To evaluate the anti-angiogenetic effect of the combination of low-dose cyclophos- phamide(CT )and ginsenoside Rg3 in mice with Lewis lung carcinoma, and to observe the anti-tumor effect, toxicity, adverse reaction of the treatment and survival timeof thetumor bearing mice.Methods Holland C57/ BL6 Lewis lung carcinoma mice were taken as the model and randomly divided into 5 groups, i.e.the low-dose CT (LDCT )group, the maximum tolerable dose CT (MTDCT )group, the ginsenoside Rg3 (Rg3) group, the low-dose CT combined with ginsenoside Rg3 group (LDCT +Rg3), and themodelgroup.Tumor volume, weight of mice, peripheral white blood cell counts and survival time of mice were observed, tumor mi- crovascular density (MVD)and vascular endothelial