年龄相关性听力损失小鼠听皮层神经元凋亡与听功能变化.pdfVIP

·457 · Chinese Journal of Otology Vol. 11, No.3, 2013 ·基础研究 · 年龄相关性听力损失小鼠听皮层神经元 凋亡及听功能变化 任红苗王宜南陈继川 第三军医大学附属大坪医院野战外科研究所耳鼻咽喉- 头颈外科（重庆400042 ） 【摘要】目的探讨年龄相关性听力损失C57BL/6J 小鼠初级听皮层神经元凋亡及听功能变化。方法选取年轻组（2 月龄）和老年组（10月龄）C57BL/6J 小鼠为研究对象，通过听性脑干反应（ABR ）检测并比较年轻组与老年组小鼠听力 变化；用HE 染色法、Tunel-POD 法观察并比较两组小鼠初级听皮层神经元大体形态及凋亡比例的差异。结果老年组 小鼠较年轻组小鼠听阈明显提高。老年组C57BL/6J 小鼠初级听皮层神经元凋亡比例较年轻组增多。结论初级听皮 层神经元的凋亡与C57BL/6J 小鼠年龄相关性听力损失密切相关。 【关键词】年龄相关性听力损失（ARHL ）老年性聋, 初级听皮层, 凋亡 【中图分类号】R764.463 【文献标识码】A 【文章编号】1672-2922（2013）03-04 ElectrophysiologicalandPathologicalChangesinC57BL/6J MicewithAge-RelatedHearingLoss RENHong-miao,WANGYi-nan,CHENJi-chuan DepartmentofOtolaryngologyHeadandNeckSurgery,KeyLaboratoryofTrauma, BurnsandCombinedInjury,InstituteofSurgeryResearch, DapingHospital,theThirdMilitaryMedicalUniversity,Chongqing,400042,China Correspondingauthor:CHENJi-chuan E-mail :chenjcmd@ 【Abstract】Objective To investigate neuronal apoptosis in primary auditory cortex and changes of auditory acuity in C57BL / 6J mice with age-related hearing loss （ARHL ）. MethodsYoung (2 months old) and aged (10 months old) C57BL / 6J mice were used to detect and compare hearing function changes by auditory brainstem responses. HE staining and Tu⁃ nel-POD methods were used to examine the morphology of primary auditory cortex neurons and compare the apoptotic rates between the two groups. Results Hearing thresholds of the aged group were significantly elevated than the young group. The number of apoptotic neurons in the primary auditory cortex of aged group increas