黄鳝主要组织相容性复合体Iα基因的克隆及多态性分析.PDFVIP

() 2015 8 41 4 Journal of Hunan Agricultural University (Natural Sciences), Aug. 2015, 41(4):396–400 DOI:10.13331/ki.jhau.2015.04.011 黄鳝主要组织相容性复合体 Iα基因的克隆及多态性分析 ( 434025)   摘 要RACE cDNA MHC IαcDNA MHC IαcDNA 1 731 bp5′UTR 78 bp3′UTR 600 bp1 350 Iα4 3 MHC IαPCR MHC Iα (Aeromonas hydrophilia)MHC Iα 42 MHC Iα3 42 50 1~6 3 IαPBR PBR d d (P0.05) N S Iα 关 键 词 中图分类号Q959.482 S966.4 文献标志码：A 文章编号：1007− 1032(2015)04−0396−05   Molecular clone and polymorphism analysis on exon 3 of MHC Class Iα gene in swamp eel, Monopterus albus Li Wei, Jiang Ao, Wu Hui (College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China) Abstract: The cloning process of full-length cDNA of MHC class Iα gene from swamp eel (Monopterus albus) was reported in the paper. The full length of cDNA, encoded with 350 amino acids, was 1 731 bp, including a 5 untranslated region (UTR) with 78 bp-long, and 3′ UTR with a 600 bp-long. The genomic sequence was identified to be different from that of in other teleosts, which contained of four exons and three introns. Real-time quantitative PCR demonstrated that the MHC class Iα gene ubiquitously expressed in ten healthy tissues. Great changes in liver, spleen and kidney were observed after they infected by Aeromonas hydrophilia, a pathogenic bacteria. Fifty distinct alleles were isolated from forty-two individuals, and each of them had one to six alleles. The presence of six alleles in one individual suggested that there were at least three MHC Iα loci in the