基质金属蛋白酶9在骨肉瘤中的表达及其意义.docVIP

基质金属蛋白酶9在骨肉瘤中的表达及其意义 作者：胡辉东,文会龙,陈金福 作者单位：常州市第四人民医院骨科，江苏 常州 213001 【摘要】 目的 研究骨肉瘤组织中基质金属蛋白酶9(MMP9)的表达情况，探讨它与骨肉瘤的关系和临床意义。方法 对29例骨肉瘤和19例正常骨组织分别石蜡切片，免疫组化了解MMP9在各组中的表达；提取两组总RNA行RTPCR，观察各组MMP9 mRNA转录改变，了解MMP9 mRNA与骨肉瘤的关系。结果 骨肉瘤组织中MMP9蛋白表达明显较正常组高，P＜0.05；正常骨组织中MMP9 mRNA的表达与Βactin比值为0.39±0.12，在骨肉瘤组织中比值为0.78±0.04，两组间比较显示，骨肉瘤组织MMP9 mRNA的表达明显升高，P＜0.05。结论MMP9在正常骨组织中呈低表达,而在骨肉瘤中的表达明显上调，MMP9与骨肉瘤的发生发展密切相关。 【关键词】 骨肉瘤；基质金属蛋白酶9；表达 　　Expression and Significance of Matrix Metalloproteinase 9 in Osteosarcoma 　　HU Huidong，WEN Huilong，CHENG Jinfu，et al 　　（Department of Orthopedics，Changzhou Fourth Renmin Hospital,Changzhou Jiansu 213001,China） 　　ABSTRACT：Objective To study the relationship between matrix metalloproteinase 9 (MMP9) mRNA expressions in osteosarcoma and its clinical significance.Methods The expressions of MMP9 were known in 29 cases of osteosarcoma and 19 cases of normal bone tissues specimens by RTPCR.To observe the change of expressions of MMP9 mRNA and to detect MMP9 protein expression by innunhistochemical staining for every group.Image analysis technique was applied for determing the average optical level of the expressed protein.Then determin the dependance between the MMP9 gene and the invasiveness of osteosarcoma.Results Compared with control group,the average optical level of MMP9 of osteosarcoma were higher（P0.05）.In osteosarcoma the ratio of MMP9 mRNA straps and Βactin was 0.78±0.04,there was significant difference(P0.05) in comparison with 0.39±0.12 in normal bone tissues.The expressions of MMP9 mRNA in osteosarcoma were increased.Conclusion The expression of MMP9 in osteosarcoma was markedly higher than in normal bone tissues.MMP9 can promote the devolepment of the osteosarcoma. 　　KEY WORDS:Osteosarcoma;Matrix metalloproteinase 9;Expression 　　骨肉瘤是常见于青少年的恶性肿瘤,具有侵袭力强、转移早且转移率高等特点。血管生成是骨肉瘤生长和转移的关键步骤。近年来的研究表明,基质金属蛋白酶类(matrix metalloproteinases,MMPs) 与血管生成的关系十分密切。MMP9 是MMPs中较重要的一种,是降解基底膜的主要酶类。M