心室纤颤时心肌缝隙连接蛋白Cx43的变化.doc

宇国危重病急救医学2010年10月第z2卷第10期Chin Crit Care Med，October 2010，V01．22，No．10 · 595· · 论著· 心室纤颤时心肌缝隙连接蛋白Cx43的变化 刘红臻 钟敬泉 李景莎 苏国英 王静 张运 【摘要】 目的 观察心室纤颤(室颤)发生后缝隙连接蛋白cx43的表达以及缝隙连接改造剂zPl23对 Cx43表达的影响。方法按照随机数字表法将30只家猪分为假手术组、模型组和ZPl23干预组，每组10只。 以80 V电压持续刺激动物5 s诱发室颤；致颤前15 rain ZPl23组给予ZPl23 1 t-g／kg静脉推注+ZPl23 10 pg· kg_1· h“微量泵泵入；模型组泵入生理盐水50 ml；假手术组动物不致颤也不补液。室颤持续8 min 后开胸取左心室游离壁心肌，用免疫荧光结合激光共聚焦显微镜技术检测Cx43的分布及水平，用蛋白质免 疫印迹法(Western blotting)定量检测Cx43蛋白表达。结果假手术组Cx43荧光信号强，分布均匀；模型组 Cx43荧光信号弱，呈不均一分布；ZPl23干预组Cx43荧光信号增强，不均一分布减轻。与假手术组比较，模型 组心室肌组织Cx43荧光信号面积百分比、积分吸光度(A)值及蛋白表达均明显下降[面积百分比：(o．64士 0．36)％比(1．27士0．19)％，积分A值：15 201士2 613比30 634士4 975，Cx43蛋白表达：0．72士0．08比 0．97士0．07，均P0．053；与模型组比较，ZPl23干预组Cx43表达[面积百分比(0．96士0．16)％，积分A值 2Z 100士4 404，Cx43蛋白表达0．82士0．043均明显升高(均PO．05)。结论室颤发生时心肌组织Cx43表 达减少；应用ZPl23可减少或逆转Cx43的降解。 【关键词】心室纤颤；缝隙连接蛋白Cx43； 缝隙连接改造剂ZPl23 Changes in myocardial connexin 43 during ventricular fibrillation LIU Hong-zhen，ZHoNG Jing— quan， LI Jing— sha，SU Guo一∥ng，WANG Jing，ZHANG Yun．Department ofCardiology，Qlu Hospital Affiliat— ed to Shandong University，Jinan 250012，China Corresponding author：zHONG Jing— quan，Email：zwkyl668@vip．sina．coin [Abstract]Objective To observe changes in connexin 43(Cx43)after ventricular fibrillation(VF) and the effects of rotigaptide(ZPl23)on Cx43．Methods Thirty domestic pigs were randomly assigned to three groups(10 in each group)：sham group，model group and ZPl23 group．VF was induced by an 80 V AC transthoracic shock for 5 seconds with the use of subcutaneous needles．Before the induction of VF。 animals in ZPl23 group were administered with ZPl23(1 ttg／kg bolus+10 pg· kg一1· h一1 dissolved in 50 ml normal saline and pumped for 15 minutes)．Those in model group received 50 ml normal saline pumped for 15 minutes．For pigs in sham group VF was not induced and no fluid was given．After 8 minutes of VF。 animals were euthanized and myocardial tissues were harvested along the long axis of each left ventricular free wall．Immunofluorescence combined with laser scanning confocal