体视显微镜法观察烤烟花芽分化的研究.pdfVIP

φ 喃-tt (盖.ðl 2011 ,27(03):143-146 Chinese Agricultural Science Bul1etin 体视显微镜法观察烤烟花芽分化的研究 段.I.棋1 ，普艳1 ，杨宇虹1 ，企磊2 ，周冀街3 ，黄成江4 (1云南省烟草农业科学研究院，云南R溪 653100;2阿南省炯单:公司济源市公司，向南济源454650; 湖南农业大学烟尊科学与健康震点实验寞，长沙410128;4云南省烟草公司，昆明 65ω11) 摘要:对低温诱导后的烤烟菜端分生组织花芽分化过程进行了体视显微镜法观察，镜检结果表明，该方 法比扫描电镜法和石蜡切片法再有明显的简便性和快捷性。此方法对菱端部位的空间特征的凸显性优 于扫描电镜的方法，具有较好的整体性;同时与扫描电钝法观感到的烤烟侧桂花序分化相一致，这是石 蜡切片法难以捕捉和发现的。 关键词:烤烟;体视;在微观察;花芽分化 中图分类号:S572 文献串串忠惆:A 论文编号:2010-2193 Studyo侧ntl伽le Floral 肌Difπferentia削硝tio佣nofF凹lue-盼←←←.销唰唰咄翩蛐-咄. Duan Yu呵E呵q旷i\ Jin Y时， Yang Yuhong\ Jin Leí. 2Zhou Jihe时. HuangChen忍jiang‘ (1 Yunnan Academy ofTo加cco AgricuJtura1 Scùmce, Yuxi Yunnan 6531峨 2Jiyuan Company ofHenan 岛阳cco CorporatÍon, Jiyuan Henan 454650; 3Resesreh Center of Tobacco Science and H.刷地Hu1l/UJ A伊cultU18.i Universi纱，， Cha吨sha410128; Yunnan To缸cco Corporation, Kunming 65ω11) Abstract: The floral hud differentíation processes on apical meristem of flue-cured tohacco inducecl with low temperature were ohserved hy stereomicl1栅cope. The results showed that this method w舶 simpler and faster 也an that of scanning electron microscope and paraffin section. lt revealed that the stereomicroscope is more superior in showing the spatial characteristics of meristem than that of the scanning electronic microscope method , and the image is more complete. It also validated the uniqueness of the inflorescence initiatíon of flue-cured tohacco lateral sh∞，t which w幽 ohserved hy scanning electron microscope , and 出is uni甲leness could not he captured or found hy paraffin section. Key words: flueωcured tohacco; stereomicroscope ohservation; flower hud differentiatÍon o sl1驾