血液透析室透析液及透析用水的细菌学监测.pdfVIP

中华医院感染学杂志2004 年第 14 卷第 4 期 4·03 · ·论 　著· 血液透析室透析液及透析用水的细菌学监测 李 　洁 , 吴安华 , 黄 　昕 , 王曼平 , 冯 　丽 , 龚瑞娥 ( 中南大学湘雅医院 , 湖南 长沙 410008) 摘要 : 目的 　了解血液透析室透析液及透析用水细菌污染的原因及对策。方法 　检测血液透析液及透析用水中 细菌的含量及细菌种类。结果 　透析器入口处透析液的平均菌落数为 486 CFU/ ml ,透析器出口处透析液的平均 菌落数为 490 CFU/ ml ,反渗水的平均菌落数为 103 CFU/ ml ,常见细菌为枯草杆菌、类白喉杆菌及某些革兰阴性 杆菌。结论 　透析液在配制的过程中 ,有多种环节可能造成污染 ,应加强透析液的监测工作 ,并建议修改透析液 细菌监测的标准。 关键词 : 透析液 ; 细菌 ; 污染 ; 监测 中图分类号: R459 5 　　文献标识码 : A 　　文章编号 : (2004) Microbial Monitoring of Dialysis Fluid in A Hemodialysis Unit L I Jie , WU Anhua , HUAN G Xin , WAN G Manping , FEN G Li , GON G Ruie ( ) Xian gya Hospital , Cent ral South U niversity , Changsha 410008 , China Abstract : OBJECTIVE To evaluate the levels of bacterial contamination in dialysis fluid in a hemodialysis unit ,and analyze the causes of contamination and strategies for the prevention of contamination. METHODS The levels of bacterial contamination and species of bacteria isolated from dialysis fluid were detected. RESULTS The average bacteria colony forming unit per milliliter in dialysis fluid from the dialyzer inlet , outlet and reverse osmosis was 486 CFU/ ml , 490 CFU/ ml and 103 CFU/ ml , respectively. The common isolated bacteria were B acillus subtilis , diphtheroid bacilli and some Gramnegative bacilli. CONCL USIONS During the process of preparation of dialysis fluid , many factors can cause microbial contamination in dialysis system ; strict monitoring of dialysis fluid should be made. Key words : Dialysis fluid ; Bacteria ; Contamination ; Monitoring 　　血液透析系统由水处理系统、混合透析用水和