稀释果汁中厌氧菌的检测方法(国外英文资料).docVIP

浓缩果汁中厌氧菌的检测方法(国外英文资料) Qixia sea rose Fruit industry Co., Ltd. Company name: Qixia Haisheng Fresh Fruit Juice Co., Ltd. Title: detection method of anaerobic bacteria number: QIXHS/ISO C - JG - 061-061 A total of 31 pages 16 revision status: 0 The detection method of anaerobic bacteria 1 scope This method is suitable for the detection of anaerobic bacteria in concentrated juice. 2 introduces Butyric acid anaerobic bacteria, such as anaerobic nitrogen-fixing bacteria clostridium on behalf of the pap, is a kind of strict anaerobic bacteria, have been able to separation of test from the metamorphism of concentrated juice, so sometimes we can test from the concentrated juice anaerobic bacteria spore. The number of bacteria is small, so the sample size is larger. 3 equipment and materials The thermostatic water bath pot A thermostat incubator 3.3 electronic balance 3.4 high-pressure steam sterilizer 3.5 membrane filtration unit 3.6 anaerobic can: volume 2.5 L 3.7 sterilized glass sample bottle: 250ml 3.8 vacuum pump 3.9 the alcohol thermometer: 0-100 ℃ 3.10 the timer 3.11 tweezers 3.12 alcohol lamp 3.13 microbiological examination membrane (hereinafter referred to as microexamination membrane) : 0.45 um 3.14 anaerobic indicator 3.15 anaerobic air generator: 2.5 L Anaerobic tank vacuum pump 4 culture medium and reagent Orange serum AGAR medium: in appendix 2, paragraph 16. 4.2 75% ethanol Anhydrous alcohol 4.4 sterilized distilled water 5 steps Preparation of sample 5.1 The 50g samples were taken from the 250ml glass sample bottle in aseptic method, and the same number was used as a temperature control sample. 5.2 thermal shock Figure 12 5.2.1 will sample bottles to insert a thermometer, temperature control with the samples at the same time in the 60 ℃ water bath pot (note: the water-bath water level must be higher than that of sample liquid level). For the temperature control in the sample bottle thermometer reaches 60 ℃ start timing, timing for 10 min (figure 12). 5.2.2