Co射线与EMS对小麦近等基因系TcLr10的复合诱变-华北农学报.PDF

华北农学报2008, 23( 增刊) : 9295 60Co EMS TcLr10 程志锋, 杨文香, 刘大群 ( , , 071000) 60 : Co EMS TcLr10 , 60 , Co EMS , ,, 0. 15 kGy , EMS 60 0.4% , Co 0. 15 kGy , 3 EMS 60 0.4% , Co 0. 15 kGy :; ; ; EMS :S435. 121.4 3 :A : 1000- 7091(2008) - 0092- 04 Compound Mutagenesis on Nearisogenic Lines TcLr10 by 60Co Ray and EMS in Wheat CHENG Zhifeng, YANG Wenxiang, LIU Daqun ( Department of Plant Pathology, College of Plant Protection,Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China) 60 Abstract: Co ray and EMSwere usedto treat wheat leaf rust resistance gene lineTcLr10 in order to establish the compound mutagenesis system for wheat near isogenetic line.The germination rate, trefoil stage,fatal rate and disease re sistance after treatment were investigated.The result showed that the effect of germination rate was not significant affected 60 by the treatment of Co ray and EMS at different dosage.The wheat was nearly reach trefoil stage at the same time in every mutagenesis dose.However, the time required from trefoil stage to quatrefoil stage was decreased with the concentra tion increase.The fatal rate remarkably increasedwhen the irradiation dose was or over 0. 15 kGy.Three mutants of near 60 isogenic linesTcLr10 inwheat were obtained when the concentration of EMS was 0.4% and the irradiation dose of Co ray was 0. 15 kGy.The effect of compound mutagenesis on Tclr10 was the best when the irradiation dose was 0. 15 kGy and the concentration of EMS was 0.4%.