takara primestar GXL 酶 操作手册.pdf

Cat. # R050A For Research Use PrimeSTAR® GXL DNA Polymerase Product Manual v1108Da Cat. #R050A PrimeSTAR® GXL DNA Polymerase v1108Da Table of Contents I. Description 3 II. Components 3 III. Storage 3 IV. Protocols 4 V. Optimization of Parameters 6 VI. Electrophoresis, Cloning, and Sequencing of Amplified Products 7 VII. Troubleshooting 7 VIII. Features 8 IX. Related Products 11 2 URL: Cat. #R050A PrimeSTAR® GXL DNA Polymerase v1108Da I. Description PrimeSTAR GXL DNA Polymerase is a revolutionary PCR enzyme that augments the high-fidelity PrimeSTAR HS DNA Polymerase by modification with a novel elongation factor to dramatically increase PCR performance. The superior performance of PrimeSTAR GXL DNA Polymerase is unsurpassed by other commercially available high-fidelity PCR enzymes. PrimeSTAR GXL DNA Polymerase allows amplification of products ≧ 30 kb in length while maintaining exceptionally high fidelity. Suitable for GC-rich templates that are otherwise difficult to amplify, this enzyme enables successful amplification from challenging templates without need for extensive optimization of buffers or reaction conditions. In addition, PrimeSTAR GXL DNA Polymerase is compatible with a wide template quantity range, and is capable of robust amplification even in the presence of large excesses of non-target DNA. Excess non-tar