树突状细胞的电离辐射效应及相关机制研究进展-第二军医大学学报.doc

电离辐射对树突状细胞的影响 杨彦勇， 蔡建明 * 第二军医大学海军医学系防原医学教研室，上海 200433 作者简介：杨彦勇，硕士，E-mail：yyyang2010@163.com 通讯作者（Corresponding author）：蔡建明， Tel：021E-mail：cjm882003@ [摘要] 电离辐射可以导致机体免疫功能低下，对人员健康及放射病的治疗及预后有重要影响。树突状细胞（Dendritic Cells，DCDC的影响不仅可以为辐射导致免疫抑制的机制提供依据，也可以通过改善照后DC的功能状态进行辐射防护研究，基于DC的癌症疫苗联合放射治疗也为肿瘤的治疗提供了新的思路。本文综述了电离辐射对DC存活率、表型、抗原提呈功能和迁移能力、分泌细胞因子的影响，以及这些辐射生物效应产生的分子机制，同时也探讨了DC疫苗结合放疗在肿瘤治疗中的一些研究进展。旨在从免疫学的角度为辐射防护提供新的思路，也为改善受照人员免疫功能状态提供文献支持。 [关键字]：树突状细胞，电离辐射，免疫抑制，肿瘤治疗 Advances in Effects of Ionizing Radiation on Dendritic Cells YANG Yanyong, CAI Jianming * Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433 [Abstract] Ionizing radiation (IR) often causes immune dysfunction, which result in severe damages to human health and affect the recovery of radiation sickness. Dendritic cells (DC) are the most potent antigen presenting cells (APC), which are also the initiator and modulator of immune system. Studies on the radiation effects of DC provide evidence into the mechanism of radiation induced immunosuppression, but also can we improve DC function for radioprotection. DC based cancer vaccine combined with radiotherapy also provide new insights into cancer therapy. This paper reviewed the influence of IR on DC viability, phenotype, migration capacity and antigen presenting function, cytokines secretion, as well as the underlying mechanism. We also reviewed some advances in tumor therapy combined of DC vaccine and radiotherapy. It is to provide literature evidence for radiation induced immunosuppression and radioprotection. [Key words]: Dendritic cells, Ionizing radiation, Immunosuppression, Cancer therapy 电离辐射导致的机体免疫功能低下对急、慢性放射病和感染的发生发展有重要影响。电离辐射除了杀伤辐射敏感细胞外，还对辐射抵抗细胞的功能有一定影响。树突状细胞（Dendritic Cells，DC[1-4]。但是国内外研究表明，不同剂量照射下，DC的表型、功能、生存状态会发生一定程度的改变，因而影响其免疫功能的发挥。 1、电离辐射对DC存活率的影响 DC是一种辐射抗性比较高的细胞，体外实验中，不同剂量的X射线或Gamma射线照射并不导致DC凋亡或坏死[2, 5-7]。但是紫外线却可以使上皮组织中的朗格汉斯细胞（Langehans cell，LC）明显减少，而下游淋巴组织的LC未见增多[8]。同时光化学疗法也可以导致DC凋