大鼠白细胞介素6(IL-6)ELISA试剂盒说明书(国外英文资料).docVIP

大鼠白细胞介素6(IL-6)ELISA试剂盒说明书(国外英文资料) The rat leucocyte 6 (il-6) enzyme-linked immunoassay Use the instruction manual for the kit Xiamen huijia biotechnology co., LTD This reagent is used only for research purposes: this kit is used to measure the rat serum, plasma, cell supernatant liquid and related samples levels of interleukin 6 (IL - 6). The experimental principle: The experimental formulae were used to determine the level of interleukin 6 (il-6) in the specimen. With purified antibody of interleukin 6 pack rats by microporous plate, made from solid phase antibody, the package in the sheet resistance of the microporous, in turn, to join the interleukin 6, and combined with HRP labeled goat anti mouse antibody, antibodies - antigen - enzyme mark complex formation, thoroughly with the substrate TMB color after washing. TMB is converted into blue by the catalysis of HRP, and is converted to the final yellow by acid. The depth of the color is positively correlated with il-6 in the sample. The absorbance (OD) was determined at 450nm wavelength by the enzyme marker, and the concentration of interleukin 6 (il-6) was calculated by the standard curve. Kit: Kit of 48 hole configuration 96 hole configuration save The instructions 1 1 Sealing plate membrane 2 slices (48) 2 (96). bags 1 1 Enzyme standard package is plate 1 x 48 1 x 96 2-8 ℃ save Standard: 180 ng/L 0.5 ml x 1 bottle 0.5 ml x 1 bottle 2-8 ℃ save Standard diluent 1.5 ml x 1 bottle 1.5 ml x 1 bottle 2-8 ℃ save Enzyme standard reagent 3 ml x 1 bottle 6 ml x 1 bottle 2-8 ℃ save The sample diluent 3 ml x 1 bottle 6 ml x 1 bottle 2-8 ℃ save A liquid agent 3 ml x 1 bottle 6 ml x 1 bottle 2-8 ℃ save Chromogenic agent B liquid 3 ml x 1 bottle 6 ml x 1 bottle 2-8 ℃ save Termination of the liquid 3 ml x 1 bottle 6 ml x 1 bottle 2-8 ℃ save Concentrated liquid detergent 20ml times 20 times a bottle 20ml times 30 times a bottle 2-8 ℃ save Sample processing and requirements: Serum: the blood naturally coagulated at room temper