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猪血液的实验室检测及数据分析技术(国外英文资料)
猪血液的实验室检测及数据分析技术(国外英文资料)
Laboratory testing and data analysis techniques for pig blood
The ministry of agricultures quality supervision and inspection and testing center (guangzhou) liu jianying, cao changren of the ministry of agriculture (guangzhou)
preface
The detection of hematology is becoming more and more important in current pig production. Provide a more scientific basis for guiding pig production, to provide more accurate evaluation standard for vaccination, provide the methods of raising management and change with the detailed data and laboratory test results of test and data analysis technology is more and more attention by the scientific and technological workers and producers, this paper in brief, to the topic.
Collection of blood samples
The blood collection of a pig is selected in the ear vein, the jugular vein and the anterior cavity vein. Although it is convenient to extract blood from the ear vein, the blood supply is insufficient. Before the jugular vein and vena cava to the technical requirement of the blood is higher, generally difficult to master, but once mastered skills, the speed of blood will very quickly, often within a few seconds to complete.
Used to directly collect blood generally use a syringe, but blood is vulnerable to pollution, easy to cause the red blood cell rupture and problems of blood lipid, greatly affect test results.
The blood protection fluid is recommended to collect blood, the specific step is: to collect the blood protection fluid by the syringe about 2 ml, and collect the blood about 2 ml, mildly blend. The blood samples collected at this time were colorless, not pale yellow, and the blood samples were greatly beneficial for ELISA, PCR, and blood physiological biochemical tests.
Before conducting a blood test, determine the sample size. According to the principle of biometrics, the method of stratification sampling should be adopted. The first is to identify a group of people, such as the public hogs, the mothers and
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