现代生物学实验习题(国外英文资料).docVIP

现代生物学实验习题(国外英文资料) The document comes from the network, it is oneself collect to organize, if have omission, mistake, still ask everybody to correct! Modern biology experiment Each experiment shall complete an experiment report in the format of laboratory report paper (2) to answer the relevant questions in each module The puzzle summary: Part one: biochemical experiments First module: sugar experiment technology How to choose the experimental materials in biochemical experiments? What is the standard? What are the broken methods of materials? Does the basic copper reagent method determine whether the original sugar is titrate or indirect titration? What are the advantages of two methods of titration? 4, what factors are the main factors influencing the measurement of crude fibers? Why do you have to strictly control the experimental conditions in the measurement of coarse fibers What is the result of this method? Module 2: enzyme experimental technology 1, why does the enzymes specificity test do the reagent test? What might be the result of skipping this step? What is the specificity of enzyme action? Try to show the effect of reversible inhibitors and irreversible inhibitors What is the relationship between the inhibition of three reversible inhibitors and the concentration of substrate? What is the molecular mechanism for the loss of enzyme activity due to high temperatures? How do you overcome this effect in the purification of enzymes? What other factors can lead to lower enzyme activity? What is the molecular mechanism for the loss of enzyme activity by pH? The pH value change causes the loss of activity, and can the pH return after recovery? The physical and biological significance of the meters constant Km Why is it that the meter constant Km is a characteristic constant of the enzyme and the Vm is not? Please turn the enzyme that has been done into a quantitative experiment Module 3: protein experimental technology Why do you use 0.145 mol/L TCA to extract