细胞计数板应用方法(国外英文资料).docVIP

细胞计数板使用方法(国外英文资料)Sit still for a moment, and let the cells settle onto the counter, no longer drifting with the liquid. The cell count plate is placed on the platform of the microscope, and then the counting area is found in the low magnification and then the high magnification is observed and counted. Because the refractive index of living cells is similar to that of water, the intensity of illumination should be reduced by observation.The count area is composed of 16 large squares, and the number of bacteria in the four large squares on the left, left, right, and right is in the direction of the diagonal. If it is a counting area of 25 large squares, all four of these are extra generous, and the number of bacteria in the central box (i.e. 80 small cells) is required. In order to ensure the accuracy of the count, avoid duplicates and omission, in the count, the statistics of the cells deposited on the grid should be uniform. If the microbe is located on the double line in the large square, the count can be counted without counting the line, and the left line is not to the right line to reduce the error. The cells in this grid and left line are included in this grid, and the cells in the lower and right lines of this grid are included in the corresponding lattice. See the figure below: the cell in this cell is three.In the case of budding yeast, the buds will be evaluated as two bacteria in half the size of the mother cell. The number of cells per mL (g) is calculated by using the formula to calculate the number of cells per mL (g).When measuring, remove the cover glass and rinse the cell counting board with water. Do not wash or wipe with hard material to avoid damage to the grid scale. After washing, air dry or dry with a hairdryer and put into the box.The cell count plate - the counting formulaThe cell counting board of 16 g / 25 cells calculates the formula: cell count/ml = 100 microcell count / 100 times 400 by 10000x dilutionThe cell counting board of 25 g / 1