异常黑胆质成熟剂对异常黑胆质载体动物模型下丘脑-垂体-肾上腺轴的调节作用研究.docVIP

异常黑胆质成熟剂对异常黑胆质载体动物模型下丘脑-垂体-肾上腺轴的调节作用研究 【摘要】 目的阐明异常黑胆质成熟剂对异常黑胆质载体动物模型丘脑-垂体-肾上腺轴(HPAA)功能的调节。 方法 根据维吾尔医学 理论 采用多因素复制小鼠异常黑胆质载体动物模型，并用异常黑胆质成熟剂不同剂量（8.58,17.16,34.32 g/kg）， 14 d全程干预给药,此后用ELISA法检测血清ACTH和CORT含量；采用高效液相-荧光检测 分析 法分析了异常黑胆质载体动物模型小鼠血清和脑去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、3，4二羟基本乙酸（DOPAC）等单胺类神经递质含量。结果异常黑胆质成熟剂不同剂量可以降低异常黑胆质载体动物模型血清NE含量（P0.01），增加血清DA、5-HT和DOPAC含量（P0.01），同时发现异常黑胆质成熟剂能降低异常黑胆质载体动物模型脑组织中NE含量（P0.01），异常黑胆质成熟剂大剂量能增加DA含量（P0.05），小剂量和大剂量能增加DOPAC含量（P0.01）。结论提示异常黑胆质成熟剂可能通过参与神经内分泌系统的内在调节，改善异常黑胆质载体动物模型的丘脑-垂体-肾上腺轴(HPAA)功能的紊乱。 【关键词】 异常黑胆质成熟剂 载体模型 丘脑-垂体-肾上腺轴 调节 　　Abstract：ObjectiveTo study the regulative effect of Abnormal Savda Munziq (ASMq) on Hypothalamic-Pituitary-Adrenocortical axis dysfunction on Abnormal Savda carrier animal models. MethodsMulti-complicated induction methods were adopted to prepare the Abnormal Savda carrier animal models. Animals were administered with ASMq at 8.58,17.16,34.32 g/kg b. w. respectively for fourteen days in order to block the procedure of Abnormal Savda development. The concentrations of andreno corticotropic hormone (ACTH), cortisol (CORT) in serum were measured by ELISA method. HPLC-fluorescence detection methods were applied to measure the concentrations of NE, DA, 5-HT and DOPAC. ResultsAll doses of ASMq used in our experiment significantly decreased the concentration of serum NE (P0.05) and increased the concentrations of serum DA, 5-HT and DOPAC (P0.05). Additionally, NE in brain tissue was inhibited (P0.01) while the DA (P0.05) and DOPAC (P0.01) were enhanced by the administration of ASMq. ConclusionThe ASMq has regulative effects on Hypothalamic-Pituitary-Adrenocortical axis（HPAA）in the Abnormal Savda carrier animal model. 　　Key words：Abnormal Savda Munziq; Carrier animal model; HPAA; Regulation 近年来对异常黑胆质病证的 研究 表明，异常黑胆质常引起肿瘤、糖尿病、高血压病和哮喘等复杂性疾病。异常黑胆质病证与下丘脑-垂体-肾上腺轴(HPAA)功能紊乱密切相关，HPAA功能紊乱可能是异常黑胆质证的生成及导致复杂性疾病的物质基础。我们推测临床有效的异常黑胆质成熟剂可能通过调节机体HPAA功能的紊乱达到防治异常黑胆质性病证的发生 发展 之功效，HPAA可能是异常黑胆质成熟剂的作用靶点之一。本研究 应用 异常黑胆质成熟剂不同剂量14 d全程干预异常黑胆质载体动物