王不留行总黄酮提取工艺及抗氧化活性研究.pdfVIP

WWW. zgys. org 研究抢文 王不留行总黄嗣提取工艺及抗氧化活性研究 齐梁坦黄月维张航郑鸿娟杨文林黄锁义(右江民族医学院广西百色533(00) 摘 要 目的:通过单因素试验和正交试验，研究料液比、pH、酶用量和酶解温度对玉不留行总黄嗣提取率的影响。方法:采 3 用紫外分光光度法、Fenton 反应法和普鲁士兰法分别测定其清除DPPH. 和 .0日的作用及还原 Fe + 的能力，以 2 ， 6-二叔丁基 对甲革命(BHT) 为对照进行抗氧化活性试验，根据试验结果评价玉不留行总黄翻体外抗氧化活性。结果:纤维素酶提取玉不留 行总黄翻的最佳工艺条件为:料液比 1 : 30 (g : ml) ，酶解 pH 4.0 ，酶用量为3.0 ml ，酶解温度为 80(二，在此条件下玉不留行总 3 黄翻的提取率为1.92% ，王不留行总黄翻体外清徐 DPPH. 和 0日的作用及还原 Fe + 的能力均高于相同浓度的 2 ， 6-二叔丁 基对甲盼(BHT) 。结论:纤维素酶法提取玉不留行总黄嗣效采省时高效，玉不留行总黄嗣具有较强的抗氧化活性，为王不留 行的资源利用和开发提供依据。 关键词 王不留行;正交设计;纤维素酶;总黄朗;抗氧化性 中圄分类号:R282. 4 ,R285. 5 文献标识码:A 文章编号:l008 -049X (2016) 12-2244-04 Extraction Process and Antioxidation Activities of Total Flavonoids from Cowherb Seed Qi Liangyu ,Huang Yuewei ,Zhang Hang ,Zheng Hongjuan ,Yang Wenlin ,Huang Suoyi (Youjiang Medical University for Nationalities , Guangxi Baise 533棚， China) ABSτRACT Objective: To study the effects of material-liquid ratio , pH value , enzyme amount and enzyme degradation temperature on the extraction efficiency of total flavonoids from cowherb seed by single factor experiments and orthogonal experiments. Methods: The methods of ultraviolet spectrophotometry , Fenton reaction and Prussian blue were used to detect the DPPH • and • OH scaven- ging ahility , and the reducing capacity for Fe3 +. Butylated hydroxytoluene (BHT) as the contrast , the antioxidant activity of total fla- vonoids from cowherb seed was determined in vitro. Results: The optimum conditions of cellulose enzyme extraction for total flavonoids from cowherb seed were as follows: the mateòal-liquid ratio was 1 : 30( g : ml) , e皿ymolysis was performed with pH of 4. 0 , amount of 3m! and temperature at 80 C. U