紫薇茎段离体培养体系的优化.pdfVIP

河南农业科学,2017,46(3):112-117 Journal of Henan Agricultural Sciences doi:10.15933/ ki.1004-3268.2017.03.022 紫薇茎段离体培养体系的优化 刘　 晓,李　 卓,唐丽丹,王　 献∗ (河南农业大学 林学院,河南 郑州450002) 摘要:以矮首领紫薇当年生半木质化带腋芽茎段、硬枝茎段以及叶片为外植体,从消毒时间、启动 培养基、增殖培养基和生根培养基选择等方面进行离体培养体系的优化。 结果表明,茎段为较好的 外植体。 最佳消毒时间:带腋芽茎段用0.1%升汞处理8 min,诱导率为66.67%;带水培芽茎段用 0.1%升汞处理4 min,诱导率为65.55%;叶片用0.1%升汞处理4 min,诱导率为55.56%。 带腋芽 茎段最佳启动培养基为MS+6-BA 1.0 mg/ L+NAA0.10 mg/ L,诱导率为83.33%。 腋芽和水培 芽的最佳增殖培养基均为MS+6-BA2.0 mg/ L+NAA0.30 mg/ L。 腋芽增殖系数为2.42,芽长为 3.2 cm;水培芽增殖系数为3.21,芽长为2.1cm。 腋芽最佳生根基本培养基为1/ 2MS,最佳生根培 养基为 1/ 2MS + IBA 0.5 mg/ L,生根率为96.67%;水培芽最佳生根培养基为 1/ 2MS + IBA 0.5 mg/ L+活性炭(AC)0.1g/ L,生根率为81.25%。 关键词:矮首领紫薇;茎段;叶片;组织培养 中图分类号:S685.99　 　 文献标志码:A　 　 文章编号:1004 -3268(2017)03-0112-06 In Vitro Culture System Optimization of Lagerstroemia indica Stems LIU Xiao,LI Zhuo,TANG Lidan,WANG Xian∗ (College of Forestry,Henan Agricultural University,Zhengzhou450002,China) Abstract:One-year-old half-lignified stemswith axillary buds,hardwood stemswithhydroponicbudsand leaves of Lagerstroemia indica ‘Aishouling’ were used as explants for in vitro culture system optimiza- tion,including sterilization time,induction medium,proliferation medium and basic rooting medium.The results showed that the stems were the optimal explants.The optimal sterilization time of 0.1% HgCl : 2 stemswith axillary budswastreatedfor 8 min,and the induced ratewas66.67%;stemswith hydroponic budswas treated for4 min,and the induced rate was65.55%;leaves was treated for4 min,and the in- duced ratewas55.56%.Bytakingthestemsasexplants,thebestinductionmediumof stemswithaxillary budswas MS+6-BA 1.0 mg/ L+NAA0.10 mg/ L,with the in