一种深色型粘虫成虫体色突变体的遗传分析-应用昆虫学报.PDF

应用昆虫学报ChineseJournal ofAppliedEntomology 2014,51(4):922926. DOI: 10.7679/j.issn.20951353.2014.113 一种深色型粘虫成虫体色突变体的遗传分析* 李 栋 张云慧 李祥瑞 程登发** （中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室100193） 摘 要 【目的】 在粘虫Mythimna separate （Walker）常规饲养种群和室外监测中发现一种成虫体色突 变体，本文主要研究其遗传模式及进行表型观察。 【方法】用杂交实验的方法对该突变体进行观察 研究。【结果】 与正常型相比，该突变体的腹、翅颜色等形态特征与正常型有显著的不同；卵、幼虫的 颜色与正常型的没有明显的区别；蛹的颜色前期没什么区别，临近羽化时表现为颜色较深。正常型与突变 体正反交F 代均为正常型，F 代正常型和突变体分离比例为3.24 1，回交比例为1.18 1，F 代突变体 1 2 ︰ ︰ 2 自交，后代全为突变体。【结论】 该突变体的遗传符合孟德尔的遗传规律，属于常染色体上单基因控制 的隐性遗传性状。本项研究结果可以为粘虫的遗传防治、进化上的环境适应性及内在分子机制研究提供参 考。 关键词 粘虫，体色突变体，遗传分析 GeneticanalysisofaMythimnaseparate(Walker)bodycolormutant LIDong ZHANGYun-Hui LIXiang-Rui CHENGDeng-Fa** (InstituteofPlantProtection,ChineseAcademyofAgricultura Sciences,Beijing 100193,China) Abstract [Objectives] Body color mutants ofMythimna separate (Walker) were found in a laboratory colony and in the wild and their phenotypes observed. [Methods] A hybridization experiment was used to analyze the genetic basis of this mutation. [Results] Comparedwithnorma moths,theadultbodyofmutants,especia ytheabdomenandwings,weredarker incolor.Therewerenoobviousdifferencesintheappearanceofeggs,larvaeandearlypupae,butimmediatelybeforeeclosion, mutantpupaewere darkerincolorthannorma pupae. Theresults ofthehybridization experimentshowthattheproportion of the norma type in F progeny of reciproca crosses between norma and mutant parents was 100%,whereas the ratio of F1 2 segregationbetween norma andmutant types was 3.24 ︰1.Theratio inbackcrosses was 1.18 ︰1.In addition,progenies of self-crossed F mutants were exclusively of mutant type. [Conclusion] The results of the hybridization experiment are2 consistentwith Mendelian, monogenic autosoma recessiv