水稻短根突变体ksr基因的精细定位-ricescience.doc

Genetic Analysis and Gene Mapping of Short Root Mutant Rice ksr1 Ning Yong-qiang, Ding Wo-na, Zhu Shi-hua, Yu Hang-wei, Yu Hang, Lu Kai-xing (Laboratory of plant molecular biology, Ningbo University, Ningbo 315211, China) Abstract: A short root mutant ksr1 with the Kasalath background was isolated from an EMS-mutagenized population in rice. The root length of 6-day-old ksr1 seedlings was only about 20% of the wild type. Genetic analysis indicated that the short root phenotype of ksr1 was controlled by a recessive mutation in a single nuclear-encoded gene. To map the ksr1 mutation, an F2 population was generated by crossing the ksr1 mutant with Nipponbare. The KSR1 locus was linked to the SSR marker RM1223 on chromosome 4. Eight new SSR markers and two InDel markers were developed around this marker. KSR1 gene was further mapped to a 155 kb region, flanked by the InDel marker 4-24725K and the SSR marker RM17182. Key words: rice; short root; gene mapping; molecular marker The root is the major organ of plants to absorb water and nutrients from soil. Roots can synthesize amino acids, plant hormones and other bioactive substances, such as IAA, ABA and acetylcholine. These substances not only participate in root development, but also regulate shoot growth and development. Moreover, roots can secrete organic acids, enzymes, alkaloids and so on, which affects the absorption of minerals from soil as well as types and amount of microorganisms in the rhizosphere soil. Root length, as one of the most important parameters of root morphology, affects various important characters, such as nutrient uptake, lodging tolerance, drought tolerance and yield. Its role on crop yield and stress resistance has become an important field of crop breeding and been attracting more attention. Root elongation is determined by two successive processes, cell division and cell elongation. In recent years, several short root mutants of rice have been isolated and three genes, including GLR3.1, Os