绣线菊内生真菌的分离及对植物病原菌的抑制作用.PDF

安徽农业大学学报, 2013, 40(6): 975-980 Journal of Anhui Agricultural University 网络出版时间：2013-10-30 8:57:48 [URL] /kcms/detail/34.1162.S0857.002.html 绣线菊内生真菌的分离及对植物病原菌的抑制作用 * 徐庆庆，曾现银，柏 钰，花日茂 ，操海群，吴祥为，李学德，唐 俊 (安徽农业大学资源与环境学院，安徽省农产品质量安全省级实验室，合肥 230036) 摘 要：采用组织分离法，以 PDA 培养基为分离培养基，从绣线菊的根、茎和叶中分离获得 39 株内生真菌。 平板对峙结果表明， 21 株活性菌株对 6 种植物病原菌（辣椒疫霉病原菌、番茄枯萎病原菌、苹果腐烂病原菌、苹 果炭疽病原菌、葡萄灰霉病原菌、小麦赤霉病原菌）有不同程度的抑制作用，其中菌株 XXT10 对苹果腐烂病原菌、 苹果炭疽病原菌、番茄枯萎病原菌、小麦赤霉病原菌的抑制率分别达到 73.2% 、72.5% 、39.5%和 42.7% 。通过测得 的 ITS rDNA 序列与 GenBank 数据库中已知序列比对后该菌为链格孢属菌。生物学特性试验表明，该菌株在 28 ～ 32℃时，选择PDA 培养基，分别以乳糖和蛋白胨作为碳、氮源，酸碱度中性的条件下，菌落的生长状况最佳。 关键词：绣线菊；内生真菌；链格孢菌；生物学特性 中图分类号：S482.292 文献标识码：A 文章编号：1672−352X (2013)06−0975−06 Isolation and inhibition activities to plant pathogens of endophytic fungi from Spiraea XU Qing-qing, ZENG Xian-yin, BAI Yu, HUA Ri-mao, CAO Hai-qun, WU Xiang-wei, LI Xue-de, TANG Jun (Anhui Provincial Key Laboratory of Agro-Food Safety, School of Resources Environment, Anhui Agricultural University, Hefei 230036) Abstract: Thirty-nine endophyte isolates were obtained from healthy roots, stems and leaves of Spiraea. The results of preliminary test exhibited 21 endophytic isolates significantly antagonized six plant patho- gens(Phytophthora capsici, Fusarium oxysporum, Cytospora mandshurica, Colletotrichum gloeosporioides, Bo- trytis cinerea and Fusarium graminearum), especially the endophyte XXT10 strain. The strain XXT10 had sig- nificant effects on pathogens, and its inhibition rates to Cytospora mandshurica, Colletotrichum gloeosporioides, Fusarium oxysporum and Fusarium graminearum were 73.2%, 72.5%, 39.5% and 42.7%, respectively. In addition, the strain XXT10 was preliminarily identified as Alternaria sp. based on ITS rDNA sequence. The study on the biological characteristics exhibited that